Kohli Neha, Hugar Shivayogi M, Hallikerimath Seema, Gokhale Niraj, Kadam Krishna, Soneta Sanjana P
Department of Pediatric and Preventive Dentistry, KLE Vishwanath Katti Institute of Dental Sciences & Hospital (Deemed to be University), Belagavi, Karnataka, India.
Department of Oral and Maxillofacial Pathology and Oral Microbiology, KLE Vishwanath Katti Institute of Dental Sciences & Hospital (Deemed to be University), Belagavi, Karnataka, India.
Int J Clin Pediatr Dent. 2024 Mar;17(3):307-315. doi: 10.5005/jp-journals-10005-2796.
The aim of the study is to evaluate and compare the antibacterial efficacy and remineralization potential of acidulated phosphate fluoride (APF) gel with herbal dental gel containing and
Ethanolic extracts of and were prepared. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of these extracts were determined against and using the resazurin method and agar plate streaking method, respectively. The herbal dental gel was formulated, and its cytotoxicity was evaluated using an 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The antibacterial susceptibility of APF gel and the herbal dental gel was assessed using the agar well diffusion method and time-kill assay. The remineralizing potential was evaluated using a stereomicroscope.
Herbal dental gel showed better antibacterial efficacy as depicted by the zone of inhibition of 20 and 21 mm obtained against and respectively, compared to 11.50 and 16.50 mm zone of inhibition obtained by APF gel. The herbal dental gel also showed better remineralization potential than APF gel after a time interval of 24 and 48 hours, as depicted with a statistically significant -value of 0.0061 and 0.0219, respectively. In the cytotoxicity test, the fibroblasts showed 100% viability in the presence of both study groups.
The results of our study concluded that herbal dental gel is safe and nontoxic, having anticariogenic potential due to its good antibacterial action and remineralization potential as compared to conventional APF gel.
Kohli N, Hugar SM, Hallikerimath S, Comparative Evaluation of Antibacterial Efficacy and Remineralization Potential of Acidulated Phosphate Fluoride Gel with Herbal Dental Gel Containing and An Study. Int J Clin Pediatr Dent 2024;17(3):307-315.
本研究旨在评估并比较含[具体成分]的酸化磷酸氟（APF）凝胶与草药牙科凝胶的抗菌效果及再矿化潜力。
制备了[具体植物名称1]和[具体植物名称2]的乙醇提取物。分别采用刃天青法和琼脂平板划线法测定这些提取物对[具体细菌1]和[具体细菌2]的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。配制了草药牙科凝胶，并采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法评估其细胞毒性。采用琼脂孔扩散法和时间-杀菌试验评估APF凝胶和草药牙科凝胶的抗菌敏感性。使用体视显微镜评估再矿化潜力。
与APF凝胶分别获得的11.50毫米和16.50毫米抑菌圈相比，草药牙科凝胶对[具体细菌1]和[具体细菌2]的抑菌圈分别为20毫米和21毫米，显示出更好的抗菌效果。在24小时和48小时的时间间隔后，草药牙科凝胶的再矿化潜力也优于APF凝胶，其统计学显著p值分别为0.0061和0.0219。在细胞毒性试验中，成纤维细胞在两个研究组存在的情况下均显示100%的活力。
我们的研究结果表明，与传统APF凝胶相比，草药牙科凝胶安全无毒，因其良好的抗菌作用和再矿化潜力而具有防龋潜力。
Kohli N, Hugar SM, Hallikerimath S, 含[具体成分]的酸化磷酸氟凝胶与草药牙科凝胶抗菌效果及再矿化潜力的比较评估。一项[具体研究类型]研究。《国际临床儿科牙科杂志》2024；17(3):307 - 315。
