Milano M, Reddy J K, Prasad J D, Rao M S
Department of Pathology, Northwestern University Medical School, Chicago, IL 60611.
Cancer Lett. 1987 Dec;38(1-2):65-71. doi: 10.1016/0304-3835(87)90201-1.
The incidence of lung metastasis in rats with hepatocellular carcinomas (HCC) induced by ciprofibrate, a peroxisome proliferator and the expression of gammaglutamyl transeptidase (GGT) in the metastatic lesions was studied. HCC were induced in 75 male F-334 rats by chronic dietary administration of ciprofibrate (0.025% w/w) for 16-22 months. The incidence of lung metastasis was 25% in rats killed between 14 and 16 months which increased to 56% in rats killed between 20 and 22 months. The lung metastases were multifocal and present in both the blood vessels and parenchyma. All the metastatic foci examined for the expression of GGT by histochemical stain were devoid of this enzyme. The results of this study clearly demonstrate the malignant behavior of ciprofibrate-induced liver tumors and the absence of GGT expression in metastatic lesions a phenotypic property that is peculiar to the primary HCC induced by several peroxisome proliferators.
研究了用环丙贝特（一种过氧化物酶体增殖剂）诱导产生肝细胞癌（HCC）的大鼠肺转移发生率以及转移灶中γ-谷氨酰转肽酶（GGT）的表达情况。通过在75只雄性F-334大鼠的饮食中持续添加环丙贝特（0.025% w/w）16 - 22个月来诱导产生HCC。在14至16个月处死的大鼠中，肺转移发生率为25%，而在20至22个月处死的大鼠中，该发生率增至56%。肺转移灶为多灶性，存在于血管和实质中。通过组织化学染色检查所有转移灶的GGT表达情况，发现这些转移灶均缺乏该酶。本研究结果清楚地证明了环丙贝特诱导的肝肿瘤具有恶性行为，且转移灶中不存在GGT表达，这是几种过氧化物酶体增殖剂诱导的原发性HCC所特有的表型特征。
