Department of Molecular and Material Science, Interdisciplinary Graduate School of Engineering Science, Kyushu University, Kasuga, Fukuoka, 816-8580, Japan.
Fukuoka Dental Collage, Sawara-ku, Fukuoka, 814-0193, Japan.
J Mater Sci Mater Med. 2017 Sep 15;28(10):165. doi: 10.1007/s10856-017-5985-5.
Tissue engineering offers an alternate approach to providing vascular graft with potential to grow similar with native tissue by seeding autologous cells into biodegradable scaffold. In this study, we developed a combining technique by layering a sheet of cells onto a porous tubular scaffold. The cell sheet prepared from co-culturing human mesenchymal stem cells (hMSCs) and endothelial cells (ECs) were able to infiltrate through porous structure of the tubular poly (lactide-co-caprolactone) (PLCL) scaffold and further proliferated on luminal wall within a week of culture. Moreover, the co-culture cell sheet within the tubular scaffold has demonstrated a faster proliferation rate than the monoculture cell sheet composed of MSCs only. We also found that the co-culture cell sheet expressed a strong angiogenic marker, including vascular endothelial growth factor (VEGF) and its receptor (VEGFR), as compared with the monoculture cell sheet within 2 weeks of culture, indicating that the co-culture system could induce differentiation into endothelial cell lineage. This combined technique would provide cellularization and maturation of vascular construct in relatively short period with a strong expression of angiogenic properties.
组织工程提供了一种替代方法，通过将自体细胞接种到可生物降解的支架中，为血管移植物提供具有潜在生长能力的类似天然组织的血管移植物。在这项研究中，我们开发了一种组合技术，即将细胞片层铺在多孔管状支架上。由共培养人骨髓间充质干细胞（hMSCs）和内皮细胞（ECs）制备的细胞片能够渗透到多孔聚（乳酸-己内酯）（PLCL）支架的结构中，并在培养的一周内进一步在腔壁上增殖。此外，与仅由 MSC 组成的单层培养细胞片相比，管状支架内的共培养细胞片具有更快的增殖速度。我们还发现，与培养 2 周内的单层培养细胞片相比，共培养细胞片表达了更强的血管生成标记物，包括血管内皮生长因子（VEGF）及其受体（VEGFR），表明共培养系统可以诱导向内皮细胞谱系分化。这种组合技术将在相对较短的时间内提供血管构建物的细胞化和成熟，并具有强烈的血管生成特性表达。
