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硼氮共掺杂碳点作为检测姜黄素的灵敏荧光探针

Boron and nitrogen co-doped carbon dots as a sensitive fluorescent probe for the detection of curcumin.

作者信息

Bian Wei, Wang Xuan, Wang Yakun, Yang Haifen, Huang Jialin, Cai Zongwei, Choi Martin M F

机构信息

School of Basic Medical Science, Shanxi Medical University, Taiyuan, People's Republic of China.

College of Pharmacy, Shanxi Medical University, Taiyuan, People's Republic of China.

出版信息

Luminescence. 2018 Feb;33(1):174-180. doi: 10.1002/bio.3390. Epub 2017 Sep 15.

Abstract

In this present study, a fluorescent probe was developed to detect curcumin, which is derived from the rhizomes of the turmeric. We used a simple and economical way to synthesize boron and nitrogen co-doped carbon dots (BNCDs) by microwave heating. The maximum emission wavelength of the BNCDs was 450 nm at an excitation wavelength of 360 nm. The as-prepared BNCDs were characterized by multiple analytical techniques such as transmission electron microscopy, X-ray photoelectron spectroscopy, X-ray diffraction, and infrared spectroscopy. The synthesized carbon nanoparticles had an average particle diameter of 4.23 nm. The BNCDs exhibited high sensitivity to the detection of curcumin at ambient conditions. The changes of BNCDs fluorescent intensity show a good linear relationship with the curcumin concentrations in the range 0.2-12.5 μM. This proposed method has been successfully applied to detect the curcumin in urine samples with the recoveries of 96.5-105.5%.

摘要

在本研究中,开发了一种荧光探针来检测姜黄素,姜黄素来源于姜黄的根茎。我们采用简单且经济的方法,通过微波加热合成硼氮共掺杂碳点(BNCDs)。BNCDs在激发波长为360nm时的最大发射波长为450nm。通过多种分析技术对所制备的BNCDs进行表征,如透射电子显微镜、X射线光电子能谱、X射线衍射和红外光谱。合成的碳纳米颗粒平均粒径为4.23nm。BNCDs在环境条件下对姜黄素的检测表现出高灵敏度。BNCDs荧光强度的变化与0.2 - 12.5μM范围内的姜黄素浓度呈现良好的线性关系。该方法已成功应用于检测尿液样本中的姜黄素,回收率为96.5 - 105.5%。

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