Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan Province, 650500, China.
Civil and Environmental Engineering, Carnegie Mellon University, Pittsburgh, PA, 15213-3890, USA.
J Fluoresc. 2018 Jan;28(1):269-276. doi: 10.1007/s10895-017-2189-9. Epub 2017 Nov 7.
Nitrogen-doped carbon quantum dots (N-CQDs) were synthesized though a facile, economical and straightforward hydrothermal method by using polyacrylamide as both carbon and nitrogen sources. The as-prepared N-CQDs offered high quantum yield of 23.1%, exhibited good water solubility and fluorescence properties. Moreover, the N-CQDs can be used as effective probes for sensitive and selective detection of dopamine. Fluorescence of N-CQDs was effectively quenched after the addition of dopamine owing to dopamine would be transformed into dopamine-quinone under alkaline conditions. A good linear relationship between fluorescence quenching and the concentration of dopamine in the range 0.1-200 μM was obtained with a low detection limit of 0.05 μM. The proposed method showed high selectivity for dopamine in the presence of potential interfering species. Moreover, this method was successfully applied to the determination of DA in urine sample with satisfactory recoveries.
氮掺杂碳量子点（N-CQDs）通过简便、经济和直接的水热法合成，以聚丙烯酰胺作为碳源和氮源。所制备的 N-CQDs 具有 23.1%的高光量子产率，表现出良好的水溶性和荧光性能。此外，N-CQDs 可用作灵敏和选择性检测多巴胺的有效探针。由于多巴胺在碱性条件下会转化为多巴胺醌，因此加入多巴胺后 N-CQDs 的荧光会被有效猝灭。在 0.1-200 μM 的范围内，荧光猝灭与多巴胺浓度之间存在良好的线性关系，检测限低至 0.05 μM。该方法在存在潜在干扰物质的情况下对多巴胺表现出高选择性。此外，该方法成功地应用于尿液样品中 DA 的测定，回收率令人满意。
