Centro Cardiologico Monzino, IRCCS, 20138 Milan, Italy.
Department of Pharmacological and Biomolecular Sciences, University of Milan, 20133 Milan, Italy.
Int J Mol Sci. 2017 Sep 15;18(9):1987. doi: 10.3390/ijms18091987.
Brain-derived neurothrophic factor (BDNF) is a neurotrophin expressed in different tissues and cells, including neurons, endothelial cells, leukocytes, megakaryocytes and platelets. Modifications of BDNF in plasma and/or in serum are associated with neurodegenerative and psychiatric disorders, cardiovascular diseases, metabolic syndrome and with mortality risk. Indeed, changes in blood levels of BDNF may reflect those of its tissue of origin and/or promote pathological dysfunctions. The measurement of BDNF amount in plasma or in serum has been characterized with particular attention in the impact of different anti-coagulants, clotting duration, temperature (≤21 °C) and delay in blood sample centrifugation as well as in stability of storage. However, the influences of normothermic conditions (37 °C) and of clotting duration on BDNF levels in human serum have not been investigated yet. Here, we showed that time and temperature during serum preparation could be taken into consideration to assess the association and/or impact of BDNF levels in the occurrence of pathological conditions.
脑源性神经营养因子（BDNF）是一种在不同组织和细胞中表达的神经营养因子，包括神经元、内皮细胞、白细胞、巨核细胞和血小板。血浆和/或血清中 BDNF 的修饰与神经退行性和精神疾病、心血管疾病、代谢综合征以及死亡风险相关。事实上，BDNF 血液水平的变化可能反映其组织来源的变化，和/或促进病理性功能障碍。血浆或血清中 BDNF 含量的测量受到特别关注，以了解不同抗凝剂、凝血时间、温度（≤21°C）和血液样本离心延迟以及储存稳定性的影响。然而，尚未研究常温（37°C）和凝血时间对人血清中 BDNF 水平的影响。在这里，我们表明，在制备血清时，可以考虑时间和温度，以评估 BDNF 水平与病理状况发生的关联和/或影响。
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