硒代半胱氨酸转运RNA，硒蛋白生物合成的核心组成部分：分离、鉴定、修饰及测序
Selenocysteine tRNA, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification, and Sequencing.
作者信息
Carlson Bradley A, Lee Byeong Jae, Tsuji Petra A, Copeland Paul R, Schweizer Ulrich, Gladyshev Vadim N, Hatfield Dolph L
机构信息
Molecular Biology of Selenium Section, Mouse Cancer Genetics Program, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20814, USA.
School of Biological Sciences, Seoul National University, Seoul, South Korea.
出版信息
Methods Mol Biol. 2018;1661:43-60. doi: 10.1007/978-1-4939-7258-6_4.
The selenocysteine (Sec) tRNA population consists of two isoforms that differ from each other by a single 2'-O-methylribosyl moiety at position 34 (Um34). These two isoforms, which are encoded in a single gene, Trsp, and modified posttranscriptionally, are involved individually in the synthesis of two subclasses of selenoproteins, designated housekeeping and stress-related selenoproteins. Techniques used in obtaining these isoforms for their characterization include extraction of RNA from mammalian cells and tissues, purifying the tRNA population by one or more procedures, and finally resolving the two isoforms from each other. Since some of the older techniques for isolating tRNA and resolving the isoforms are used in only a few laboratories, these procedures will be discussed briefly and references provided for more detailed information, while the more recently developed procedures are discussed in detail. In addition, a novel technique that was developed in sequencing tRNA for identifying their occurrence in other organisms is also presented.
硒代半胱氨酸（Sec）tRNA群体由两种异构体组成，它们在34位（Um34）仅因一个2'-O-甲基核糖部分而彼此不同。这两种异构体由单个基因Trsp编码，并在转录后进行修饰，分别参与两类硒蛋白的合成，即管家型和应激相关硒蛋白。用于获取这些异构体以进行表征的技术包括从哺乳动物细胞和组织中提取RNA，通过一种或多种程序纯化tRNA群体，最后将两种异构体彼此分离。由于一些较老的分离tRNA和解析异构体的技术仅在少数实验室中使用，因此将简要讨论这些程序并提供参考文献以获取更详细的信息，而对最近开发的程序将进行详细讨论。此外，还介绍了一种在tRNA测序中开发的用于识别其在其他生物体中存在情况的新技术。
Zotero 插件