硒代半胱氨酸tRNA作为真核生物中硒蛋白生物合成的核心组成部分。

Selenocysteine tRNAs as central components of selenoprotein biosynthesis in eukaryotes.

作者信息

Park S I, Park J M, Chittum H S, Yang E S, Carlson B A, Lee B J, Hatfield D L

机构信息

Laboratory of Experimental Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

出版信息

Biomed Environ Sci. 1997 Sep;10(2-3):116-24.

PMID:9315302

Abstract

Selenocysteine (Sec) tRNAs serve as carrier molecules for the biosynthesis of Sec from serine and to donate Sec to protein in response to specific UGA codons. In this study, we describe the current status of Sec tRNAs in higher animals and further we examine: (i) the Sec tRNA population in Drosophila; (ii) transcription of the Sec tRNA in vivo (in Xenopus oocytes) and in vitro (in Xenopus oocyte extracts); (iii) the effect of selenium on the Sec tRNA population in various rat tissues following replenishment of extremely selenium deficient rats with this element; and (iv) the biosynthesis of the modified bases on Sec tRNA in Xenopus oocytes.

摘要

硒代半胱氨酸（Sec）转运RNA作为载体分子，用于从丝氨酸生物合成Sec，并响应特定的UGA密码子将Sec捐赠给蛋白质。在本研究中，我们描述了高等动物中Sec转运RNA的现状，并且进一步研究：（i）果蝇中的Sec转运RNA群体；（ii）Sec转运RNA在体内（非洲爪蟾卵母细胞中）和体外（非洲爪蟾卵母细胞提取物中）的转录；（iii）在用该元素补充极度缺硒大鼠后，硒对各种大鼠组织中Sec转运RNA群体的影响；以及（iv）非洲爪蟾卵母细胞中Sec转运RNA上修饰碱基的生物合成。