Cruz Elisa Castañeda Santa, Susanne Becker J, Sabine Becker J, Sussulini Alessandra
Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Department of Analytical Chemistry, Institute of Chemistry, University of Campinas (UNICAMP), P.O. Box 6154, Campinas, SP, 13083-970, Brazil.
, Konstanz, 78467, Germany.
Methods Mol Biol. 2018;1661:219-227. doi: 10.1007/978-1-4939-7258-6_16.
Selenium and selenoproteins are important components of living organisms that play a role in different biological processes. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is a powerful analytical technique that has been employed to obtain distribution maps of selenium in biological tissues in a direct manner, as well as in selenoproteins, previously separated by their molecular masses and isoelectric points using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). In this chapter, we present the protocols to perform LA-ICP-MS imaging experiments, allowing the distribution visualization and determination of selenium and/or selenoproteins in biological systems.
硒和硒蛋白是生物体内的重要组成部分，在不同的生物过程中发挥作用。激光烧蚀电感耦合等离子体质谱法（LA-ICP-MS）是一种强大的分析技术，已被用于直接获取生物组织中硒的分布图，以及之前通过二维聚丙烯酰胺凝胶电泳（2-D PAGE）按分子量和等电点分离的硒蛋白中的硒分布图。在本章中，我们介绍了进行LA-ICP-MS成像实验的方案，可实现生物系统中硒和/或硒蛋白分布的可视化及测定。
