Department of Animal Science, University of São Paulo, Luiz de Queiroz College of Agriculture, Piracicaba, Brazil, 13418900.
Department of Agri-food Industry, Food and Nutrition, University of São Paulo, Luiz de Queiroz College of Agriculture, Piracicaba, Brazil, 13418900.
J Dairy Sci. 2017 Nov;100(11):9048-9051. doi: 10.3168/jds.2017-12943. Epub 2017 Sep 13.
The objective of this study was to determine the contribution of corn kernel enzymes, bacteria, fungi, and fermentation end-products (main acids and ethanol) to protein solubilization during fermentation of reconstituted corn grain silage. Flint corn kernels were ground (5-mm sieve), rehydrated to 32% of moisture, and treated with no additives (control), gamma irradiation (32 kGy), gamma irradiation + fermentation end-products (1% of lactic acid, 0.3% of acetic acid, and 0.7% of ethanol, as fed), and natamycin (1% as fed). Treated grains were ensiled in nylon-polyethylene bags and stored for 90 d. Protein solubilization was calculated for each treatment and the contributions of proteolytic sources were determined. Bacterial activity was the main contributor to proteolysis (60%) followed by corn kernel enzymes (30%), whereas fungi and fermentation end-products had only minor contributions (∼5% each).
本研究旨在确定玉米籽粒酶、细菌、真菌和发酵终产物(主要酸和乙醇)在再水合玉米青贮发酵过程中对蛋白质溶解的贡献。将硬质玉米籽粒粉碎(5 毫米筛网),再水合至水分含量为 32%,并用无添加剂(对照)、γ 射线辐射(32 kGy)、γ 射线辐射+发酵终产物(乳酸 1%、乙酸 0.3%、乙醇 0.7%,进料)和纳他霉素(进料 1%)处理。处理后的谷物在尼龙-聚乙烯袋中青贮,并储存 90 天。计算了每种处理的蛋白质溶解,并确定了蛋白水解源的贡献。细菌活性是蛋白水解的主要贡献者(60%),其次是玉米籽粒酶(30%),而真菌和发酵终产物的贡献较小(各约 5%)。