Chen Ying-Chuan, Zhu Guan-Yu, Wang Xiu, Shi Lin, Du Ting-Ting, Liu De-Feng, Liu Yu-Ye, Jiang Yin, Zhang Xin, Zhang Jian-Guo
a Department of Neurosurgery , Beijing Tiantan Hospital, Capital Medical University , Beijing , China.
b Department of Functional Neurosurgery , Beijing Neurosurgical Institute, Capital Medical University , Beijing , China.
Neurol Res. 2017 Dec;39(12):1103-1113. doi: 10.1080/01616412.2017.1379241. Epub 2017 Sep 18.
Objective The therapeutic efficacy of anterior thalamic nuclei deep brain stimulation (ATN-DBS) against seizures has been largely accepted; however, the effects of ATN-DBS on disruption of the blood-brain barrier (BBB), albumin extravasation, inflammation and apoptosis still remain unclear. Methods Rats were distributed into four treatment groups: physiological saline (PS, N = 12), kainic acid (KA, N = 12), KA-sham-DBS (N = 12) and KA-DBS (N = 12). Seizures were monitored using video-electroencephalogram (EEG). One day after surgery, all rats were sacrificed. Then, samples were prepared for quantitative real-time PCR (qPCR), western blot, immunofluorescence (IF) staining, and transmission electron microscopy to evaluate the disruption of the BBB, albumin extravasation, inflammation, and apoptosis. Result Because of the KA injection, the disruption of the BBB, albumin extravasation, inflammation and apoptosis were more severe in the KA and the KA-sham-DBS groups compared to the PS group (all Ps < 0.05 or < 0.01). The ideal outcomes were observed in the KA-DBS group. ATN-DBS produced a 46.3% reduction in seizure frequency and alleviated the disruption of the BBB, albumin extravasation, inflammatory reaction and apoptosis in comparison to the KA-sham-DBS group (all Ps < 0.05 or < 0.01). Conclusion (1) Seizures can be reduced using ATN-DBS in the epileptogenic stage. (2) ATN-DBS can reduce the disruption of the BBB and albumin extravasation. (3) ATN-DBS has an anti-inflammatory effect in epileptic models.
目的 丘脑前核深部脑刺激（ATN-DBS）治疗癫痫的疗效已得到广泛认可；然而，ATN-DBS对血脑屏障（BBB）破坏、白蛋白外渗、炎症和细胞凋亡的影响仍不明确。方法 将大鼠分为四个治疗组：生理盐水组（PS，n = 12）、 kainic 酸组（KA，n = 12）、KA假刺激DBS组（n = 12）和KA-DBS组（n = 12）。采用视频脑电图（EEG）监测癫痫发作情况。术后1天，处死所有大鼠。然后，制备样本用于定量实时PCR（qPCR）、蛋白质印迹、免疫荧光（IF）染色和透射电子显微镜检查，以评估BBB的破坏、白蛋白外渗、炎症和细胞凋亡情况。结果 由于注射了KA，KA组和KA假刺激DBS组的BBB破坏、白蛋白外渗、炎症和细胞凋亡比PS组更严重（所有P值<0.05或<0.01）。KA-DBS组观察到了理想的结果。与KA假刺激DBS组相比，ATN-DBS使癫痫发作频率降低了46.3%，并减轻了BBB的破坏、白蛋白外渗、炎症反应和细胞凋亡（所有P值<0.05或<0.01）。结论 （1）在癫痫发作期使用ATN-DBS可减少癫痫发作。（2）ATN-DBS可减少BBB的破坏和白蛋白外渗。（3）ATN-DBS在癫痫模型中具有抗炎作用。
