巴西南部圈养北非羱羊（）中血源性支原体的分子筛查
Molecular screening for hemotropic mycoplasmas in captive Barbary sheep () in southern Brazil.
作者信息
Santos Leonilda C, Vidotto Odilon, Morikawa Vivien M, Santos Nelson J R, Vieira Thállitha S W J, Barros Filho Ivan R, Vieira Rafael F C, Biondo Alexander W
机构信息
Department of Sanitary Engineering, Engineering and Exact Sciences Center, Universidade Estadual do Oeste do Paraná, Foz do Iguaçu, PR 85870-650, Brazil.
Department of Veterinary Medicine, Universidade Federal do Paraná, Curitiba, PR 80035-050, Brazil.
出版信息
Vet World. 2017 Aug;10(8):924-926. doi: 10.14202/vetworld.2017.924-926. Epub 2017 Aug 15.
AIM
This study is part of an active surveillance program for monitoring animal health status in endangered species, and was conducted to screen captive Barbary sheep () for hemoplasma infection.
MATERIALS AND METHODS
A total of 12 blood samples were collected, DNA extracted and further tested by a pan-hemoplasma polymerase chain reaction protocol.
RESULTS
Animals were clinically healthy and not infested by ectoparasites. Although housekeeping gene DNA was successfully amplified, all the Barbary sheep samples tested negative for sp.
CONCLUSION
Notwithstanding the negative results, molecular pathogen surveys on Barbary sheep and other exotic wild mammals may provide insights regarding infection of endangered species caused by captivity stress in association with exposure to new pathogens worldwide.
目的
本研究是一项监测濒危物种动物健康状况的主动监测计划的一部分，旨在筛查圈养的蛮羊（）是否感染血支原体。
材料与方法
共采集12份血液样本，提取DNA，并通过全血支原体聚合酶链反应方案进行进一步检测。
结果
动物临床健康，未感染外寄生虫。尽管管家基因DNA成功扩增，但所有检测的蛮羊样本的 种检测结果均为阴性。
结论
尽管结果为阴性，但对蛮羊和其他外来野生哺乳动物进行分子病原体调查，可能会为圈养压力与全球范围内接触新病原体相关的濒危物种感染情况提供见解。
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