Pine Mariana Bozina, Gallo Raquel Bozini, da Silva Carlos Roberto Maximiano, Pezenti Larissa Forim, Domenico Fernando Campos De, Loreto Vilma, da Rosa Renata
Departamento de Biologia Geral, CCB, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brazil.
Museu de Zoologia, Instituto de Biociências, Universidade de São Paulo (USP), São Paulo, São Paulo, Brazil.
Comp Cytogenet. 2017 Mar 31;11(2):203-212. doi: 10.3897/CompCytogen.v11i2.10282. eCollection 2017.
In this paper, we present the cytomolecular analysis of a population of collected in the largest fragment of the Brazilian Atlantic forest, the Iguaçu National Park. The diploid number in males was 23 (22+X0), with two large pairs (1-2), 7 medium (3-9), 2 small (10-11) and the X chromosome of medium size. Heterochromatic blocks were evident in the pericentromeric regions of all chromosomes. Heterogeneity in the distribution of heterochromatin was observed, with a predominance of DAPI blocks. However, some chromosomes showed CMA blocks and other DAPI/CMA blocks. The 18S rDNA sites were distributed on the short arms of 5 pairs. In two of these pairs, such sites were in the same chromosome bearing 5S rDNA, and one of the bivalents, they were co-located. Histone H3 genes were found on one bivalent. The results added to the existing cytogenetic studies provided evidence of great karyotypic plasticity in the species. This pliancy may be the result of vicariant events related to the geographical distribution of different populations of .
在本文中，我们展示了对采集自巴西大西洋森林最大片段伊瓜苏国家公园的一个种群的细胞分子分析。雄性的二倍体数目为23(22 + X0)，有两对大染色体(1 - 2)、7对中等大小染色体(3 - 9)、2对小染色体(10 - 11)以及一条中等大小的X染色体。异染色质块在所有染色体的着丝粒周围区域明显可见。观察到异染色质分布存在异质性，以DAPI块为主。然而，一些染色体显示有CMA块以及其他DAPI/CMA块。18S rDNA位点分布在5对染色体的短臂上。在其中两对染色体中，这些位点位于携带5S rDNA的同一条染色体上，并且在一个二价体中，它们共定位。在一个二价体上发现了组蛋白H3基因。这些结果与现有的细胞遗传学研究一起，为该物种中巨大的核型可塑性提供了证据。这种柔韧性可能是与该物种不同种群地理分布相关的替代事件的结果。
