Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore, 54590, Pakistan.
Department of Microbiology, Government College University Faisalabad (GCUF), Faisalabad, Pakistan.
Appl Microbiol Biotechnol. 2017 Oct;101(20):7715-7728. doi: 10.1007/s00253-017-8503-2. Epub 2017 Sep 17.
This study examines the bioremediation potential and cadmium-induced cellular response on a molecular level in Candida tropicalis 3Aer. Spectroscopic analysis clearly illustrated the involvement of yeast cell wall components in biosorption. Cadmium bioaccumulation was confirmed by TEM, SEM, and EDX examination. TEM images revealed extracellular as well as cytoplasmic and vacuolar cadmium nanoparticle formation, further validated by presence of ycf1 gene and increased biosynthesis of GSH under cadmium stress. Fourteen proteins exhibited differential expression and during cellular redox homeostasis are found to involve in nitrogen metabolism, nucleotide biosynthesis, and carbohydrate catabolism. Interestingly, C. tropicalis 3Aer is equipped with nitrile hydratase enzyme, rarely been reported in yeast. It has the potential to remove nitriles from the environment. The Cd toxicity not only caused growth stasis but also upregulated the cysteine biosynthesis, protein folding and cytoplasmic detoxification response elements. The present study suggests that C. tropicalis 3Aer is a potential candidate for bioremediating environmental pollution by Cd.
本研究从分子水平上考察了热带假丝酵母 3Aer 的生物修复潜力和镉诱导的细胞反应。光谱分析清楚地说明了酵母细胞壁成分在生物吸附中的参与。TEM、SEM 和 EDX 检查证实了镉的生物积累。TEM 图像显示了细胞外以及细胞质和液泡中的镉纳米颗粒形成，这进一步通过 ycf1 基因的存在和在镉胁迫下 GSH 的增加生物合成得到验证。14 种蛋白质表现出差异表达，并且在细胞氧化还原平衡中发现它们参与氮代谢、核苷酸生物合成和碳水化合物分解代谢。有趣的是，热带假丝酵母 3Aer 配备了腈水合酶，这在酵母中很少有报道。它有可能从环境中去除腈。Cd 毒性不仅导致生长停滞，还上调了半胱氨酸生物合成、蛋白质折叠和细胞质解毒反应元件。本研究表明，热带假丝酵母 3Aer 是一种潜在的候选生物修复环境中 Cd 污染的生物。
