Moshtaghi Nikou Mahdi, Ramezani Mohaddaseh, Harirchi Sharareh, Makzoom Somayyeh, Amoozegar Mohammad Ali, Shahzadeh Fazeli Seyed Abolhassan, Schumann Peter, Ventosa Antonio
Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR, Tehran, Iran.
Department of Microbiology, Extremophiles Laboratory, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran.
Int J Syst Evol Microbiol. 2017 Oct;67(10):4221-4227. doi: 10.1099/ijsem.0.002286. Epub 2017 Sep 18.
A Gram-positive, halophilic actinobacterial strain Miq-12 was isolated from Meighan wetland in Iran. Strain Miq-12 was strictly aerobic, catalase positive and oxidase negative. The isolate grew at 12-25 % NaCl, at 30-50 °C and pH 5.5-10.5. The optimum NaCl, temperature and pH for growth were 15-20 %, 40 °C and 7.0-8.0, respectively. The cell wall of strain Miq-12 contained meso-diaminopimelic acid as diagnostic diamino acid and arabinose as whole-cell sugar. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. It synthesized cellular fatty acids of anteiso and iso-branched types, anteiso-C17 : 0, iso-C17:0, iso-C15:0, iso-C16 : 0. The major respiratory quinone was MK-9(H4). The G+C content of its genomic DNA was 72.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain Miq-12 belongs to the family Pseudonocardiaceae, constituted a separate clade, and showed the closest phylogenetic similarity to Saccharopolyspora aidingensis TRM 46074 (96.99 %) and Saccharopolyspora ghardaiensis CCUG 63370 (96.92 %). On the basis of phylogenetic analysis, phenotypic and chemotaxonomic characteristics, a novel genus and species of the family Pseudonocardiaceae, Salinifilum proteinilyticum gen. nov., sp. nov., are proposed. The type strain is Miq-12 (=IBRCM 11033=LMG 28390). We also propose that S. aidingensis and S. ghardaiensis should be transferred to this new genus and be named Salinifilum aidingensis comb. nov. and Salinifilum ghardaiensis comb. nov., respectively. The type strain of Salinifilum aidingensis comb. nov. is TRM 46074 (=CCTCCAA 2012014=JCM 30185) and the type strain of Salinifilum ghardaiensis comb. nov. is CCUG 63370 (=DSM 45606=CECT 8304).
从伊朗梅根湿地分离出一株革兰氏阳性嗜盐放线菌菌株Miq-12。菌株Miq-12严格需氧，过氧化氢酶阳性，氧化酶阴性。该分离株在12 - 25%的NaCl、30 - 50°C和pH 5.5 - 10.5条件下生长。生长的最适NaCl、温度和pH分别为15 - 20%、40°C和7.0 - 8.0。菌株Miq-12的细胞壁含有内消旋二氨基庚二酸作为诊断性二氨基酸，阿拉伯糖作为全细胞糖。极性脂类模式由双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱和磷脂酰肌醇组成。它合成anteiso和iso-分支类型的细胞脂肪酸，anteiso-C17 : 0、iso-C17:0、iso-C15:0、iso-C16 : 0。主要呼吸醌为MK-9(H4)。其基因组DNA的G+C含量为72.1 mol%。基于16S rRNA基因序列比较的系统发育分析表明，菌株Miq-12属于假诺卡氏菌科，构成一个单独的分支，与艾氏糖多孢菌TRM 46074(96.99%)和加氏糖多孢菌CCUG 63370(96.92%)显示出最接近的系统发育相似性。基于系统发育分析、表型和化学分类学特征，提出假诺卡氏菌科的一个新属和新种，即解蛋白盐生丝菌属新属，解蛋白盐生丝菌新种。模式菌株为Miq-12(=IBRCM 11033=LMG 28390)。我们还建议将艾氏糖多孢菌和加氏糖多孢菌转移到这个新属，并分别命名为艾氏盐生丝菌新组合和加氏盐生丝菌新组合。艾氏盐生丝菌新组合的模式菌株为TRM 46074(=CCTCCAA 2012014=JCM 30185)，加氏盐生丝菌新组合的模式菌株为CCUG 63370(=DSM 45606=CECT 8304)。
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