Xia Zhan-Feng, Luo Xiao-Xia, Wan Chuan-Xing, Zhang Li-Li
Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin/College of Life Science, Tarim University, Alar 843300, PR China.
Int J Syst Evol Microbiol. 2017 Mar;67(3):687-691. doi: 10.1099/ijsem.0.001696.
A novel halophilic actinomycete strain, designated TRM 46074, was isolated from Aiding salt lake in Turpan Basin, north-west China. Strain TRM 46074 was aerobic, Gram-stain-positive and grew optimally with 12 % (w/v) NaCl. The strain was observed to produce sparse aerial mycelium with long chains of spores that were non-motile and oval or spherical in shape with a rough or smooth surface; substrate mycelium was branched and well developed. The cell wall was determined to contain meso-diaminopimelic acid; the diagnostic whole-cell sugars were galactose, arabinose and ribose. The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones were MK-9(H4), MK-10(H8) and MK-10(H4). The G+C content of the DNA was 70.9 mol%. The major fatty acids were anteiso-C17 : 0, iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain TRM 46074 belongs to the genus Saccharopolyspora. Strain TRM 46074 had 16S rRNA gene sequence similarity of 99.6 % with the closest described species, Saccharopolyspora ghardaiensis H53. DNA-DNA hybridization between strain TRM 46074 and Saccharopolysporaghardaiensis H53 showed 58.6 % relatedness. On the basis of the evidence from a polyphasic study, strain TRM 46074 is considered to represent a novel species in the genus Saccharopolyspora, for which the name Saccharopolyspora aidingensis sp. nov. is proposed. The type strain is TRM 46074 (=CCTCC AA 2012014=JCM 30185).
从中国西北部吐鲁番盆地的艾丁湖分离出一株新型嗜盐放线菌，命名为TRM 46074。TRM 46074菌株为需氧菌，革兰氏染色阳性，在含12%（w/v）NaCl的培养基中生长最佳。观察到该菌株产生稀疏的气生菌丝体，带有长链孢子，孢子无运动性，呈椭圆形或球形，表面粗糙或光滑；基内菌丝体有分支且发育良好。细胞壁含有内消旋二氨基庚二酸；全细胞诊断性糖为半乳糖、阿拉伯糖和核糖。诊断性极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、磷脂酰甘油和磷脂酰肌醇。主要甲基萘醌为MK-9（H4）、MK-10（H8）和MK-10（H4）。DNA的G+C含量为70.9mol%。主要脂肪酸为anteiso-C17 : 0、iso-C15 : 0、iso-C16 : 0和iso-C17 : 0。基于16S rRNA基因序列的系统发育分析表明，TRM 46074菌株属于糖多孢菌属。TRM 46074菌株与已描述的最接近物种——加德糖多孢菌H53的16S rRNA基因序列相似性为99.6%。TRM 46074菌株与加德糖多孢菌H53之间的DNA-DNA杂交显示相关性为58.6%。基于多相研究的证据，TRM 46074菌株被认为代表糖多孢菌属中的一个新物种，为此提出新名称艾丁糖多孢菌（Saccharopolyspora aidingensis sp. nov.）。模式菌株为TRM 46074（=CCTCC AA 2012014=JCM 30185）。
