Yang Zi-Wen, Salam Nimaichand, Asem Mipeshwaree Devi, Fang Bao-Zhu, Lan Liu, Xiao Min, Wadaan Mohammed A M, Hozzein Wael N, Li Wen-Jun
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
Bioproducts Research Chair, Zoology Department, College of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia.
Int J Syst Evol Microbiol. 2018 Mar;68(3):860-864. doi: 10.1099/ijsem.0.002598. Epub 2018 Jan 30.
Strain SYSU D8010 was isolated from a desert sand sample collected in Saudi Arabia. The taxonomic position of the isolate was investigated by the polyphasic taxonomic approach. The isolate was found to be Gram-positive and aerobic. The strain was able to grow at 14-40 °C, pH 5.0-9.0 and in the presence of up to 22 % (w/v) NaCl. Strain SYSU D8010 contained meso-diaminopimelic acid as cell-wall diamino acid, and arabinose, fucose, galactose, glucose and rhamnose as the whole-cell sugars. The primary polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositolmannosides. Menaquinone MK-9(H4) was detected as the respiratory quinone; and anteiso-C17 : 0, iso-C16 : 0, iso-C15 : 0 and iso-C17 : 0 as the predominant fatty acids. Pairwise comparison of the 16S rRNA gene sequences indicated that strain SYSU D8010 had a sequence similarity of 97.8 % to Saccharopolyspora halophila YIM 90500. The genomic DNA G+C content of strain SYSU D8010 was determined to be 69.9 mol%. Based on the analyses of the phenotypic, genotypic and phylogenetic characteristics, strain SYSU D8010 was determined to represent a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora deserti sp. nov. is proposed. The type strain of the species is SYSU D8010 (=KCTC 39989=CPCC 204620).
菌株SYSU D8010是从沙特阿拉伯采集的沙漠沙样中分离得到的。采用多相分类法研究了该分离株的分类地位。该分离株为革兰氏阳性需氧菌。该菌株能够在14 - 40 °C、pH 5.0 - 9.0以及高达22 %（w/v）NaCl的条件下生长。菌株SYSU D8010含有内消旋二氨基庚二酸作为细胞壁二氨基酸，阿拉伯糖、岩藻糖、半乳糖、葡萄糖和鼠李糖作为全细胞糖。主要极性脂为双磷脂酰甘油、磷脂酰胆碱、磷脂酰甘油、磷脂酰肌醇和磷脂酰肌醇甘露糖苷。检测到甲基萘醌MK - 9(H4)作为呼吸醌；anteiso - C17 : 0、iso - C16 : 0、iso - C15 : 0和iso - C17 : 0作为主要脂肪酸。16S rRNA基因序列的成对比较表明，菌株SYSU D8010与嗜盐糖多孢菌YIM 90500的序列相似性为97.8 %。菌株SYSU D8010的基因组DNA G + C含量测定为69.9 mol%。基于表型、基因型和系统发育特征的分析，菌株SYSU D8010被确定代表糖多孢菌属的一个新物种，为此提出了新名称沙漠糖多孢菌（Saccharopolyspora deserti sp. nov.）。该物种的模式菌株为SYSU D8010（=KCTC 39989=CPCC 204620）。
