Yamagishi T, Gyobu Y, Sakamoto K, Ishisaka S, Saito K, Morinaga S, Katsuda S, Umei T, Konishi K
Department of Bacteriology and Immunology, School of Medicine, Toyama Medical and Pharmaceutical University.
Microbiol Immunol. 1987;31(9):859-68. doi: 10.1111/j.1348-0421.1987.tb03147.x.
The vegetative cells and toxic filtrates of Clostridium perfringens type C strains were injected into ligated rabbit ileal loops and the responses were observed. Out of 12 strains examined, 2 strains showed positive reaction in this test, when the vegetative cells were injected. One of these 2 strains was an enterotoxigenic and beta-toxigenic and the other was beta- and delta-toxigenic but not enterotoxigenic. Culture filtrates containing beta or delta toxin also showed fluid accumulation in the rabbit ileal loop. Histological findings of loops injected with culture filtrates containing beta toxin showed separated and effaced villi, hemorrhage in the mucosa, engorged vessels, inflammatory cell infiltration, and hyperplasia of the lymphoid tissue.
将产气荚膜梭菌C型菌株的营养细胞和毒性滤液注入结扎的兔回肠袢中，并观察其反应。在所检测的12株菌株中，当注入营养细胞时，有2株在此试验中呈阳性反应。这2株中的1株产生肠毒素且产生β毒素，另一株产生β毒素和δ毒素但不产生肠毒素。含有β或δ毒素的培养滤液在兔回肠袢中也显示出液体蓄积。注入含有β毒素的培养滤液的肠袢的组织学检查结果显示绒毛分离和消失、黏膜出血、血管充血、炎性细胞浸润以及淋巴组织增生。
