Duncan C L, Strong D H
J Bacteriol. 1969 Oct;100(1):86-94. doi: 10.1128/jb.100.1.86-94.1969.
The ability of cell extracts and culture filtrates of various strains of C. perfringens to produce ileal loop fluid accumulation and overt diarrhea in rabbits was tested. Good correlation was obtained in the ability of whole cells and a toxic factor (present in cell extracts and concentrated culture filtrates) to produce both fluid accumulation in ileal loops and diarrhea when injected into the normal ileum of the rabbit. The toxic factor was present in cell-free preparations when cells were grown in a sporulation medium, but not when they were grown in an asporogenic medium. The factor was shown to be heat labile, nondialyzable, and was inactivated by Pronase but not by trypsin, lipase, or amylase. Loss of activity occurred at pH 1.0, 3.0, 5.0, and 12.0.
对产气荚膜梭菌不同菌株的细胞提取物和培养滤液在兔中产生回肠袢液体积聚和明显腹泻的能力进行了测试。当将全细胞和一种毒性因子（存在于细胞提取物和浓缩培养滤液中）注射到兔的正常回肠中时，它们产生回肠袢液体积聚和腹泻的能力之间存在良好的相关性。当细胞在芽孢形成培养基中生长时，毒性因子存在于无细胞制剂中，但在无芽孢形成培养基中生长时则不存在。该因子表现为热不稳定、不可透析，被链霉蛋白酶灭活，但不被胰蛋白酶、脂肪酶或淀粉酶灭活。在pH 1.0、3.0、5.0和12.0时活性丧失。
