1 Department of Neurology, University of Minnesota , Minneapolis, Minnesota.
2 Department of Neurosurgery, University of Minnesota , Minneapolis, Minnesota.
J Neurotrauma. 2018 Jan 1;35(1):187-194. doi: 10.1089/neu.2017.5208. Epub 2017 Oct 16.
We present a longitudinal study of cerebral metabolism using [F]fluorodeoxyglucose (FDG) positron emission tomography (PET) in a rat model of shockwave-induced traumatic brain injury (SW-TBI). Anesthetized rats received 5 or 10 SW pulses to the right anterior lateral or dorsal frontal regions using SW lithotripsy. Animals were scanned for FDG uptake at baseline, 3 h post-injury, and 3 days post-injury, using a small animal PET/computed tomography (CT) scanner. FDG uptake at all time-points was quantified as the ratio of brain activity relative to peripheral activity in the left ventricle (LV) in the heart (A/A) for the entire brain, each hemisphere, and four cortices (motor, cingulate, somatosensory, and retrosplenial). The mixed-designed models analysis of variance (ANOVA) for the hemispheric and global FDG uptake ratio showed a significant effect of the time-of-scan (p = 0.038) and measured region (p = 6.12e-09). We also observed a significant effect of the time-of-scan (p = 0.046) and measured region (p = 2.28e-09) for the FDG uptake ratio in four cortical regions. None of the measurements (global or local) showed a significant effect for the number of SW pulses (5 or 10) or SW location (lateral or dorsal frontal regions). Our data suggest that SW-TBI causes hypermetabolism on the impact side of the rat brain at 3 h post-injury compared with the baseline measurements. However, the increase in FDG uptake by day 3 post-injury was not significant. Further studies on post-TBI metabolic changes are needed to understand better the pathophysiology of the injury.
我们进行了一项使用 [F]氟脱氧葡萄糖（FDG）正电子发射断层扫描（PET）的纵向研究，以研究冲击波诱导的创伤性脑损伤（SW-TBI）大鼠模型中的脑代谢。使用 SW 碎石术对麻醉大鼠右侧前外侧或额前背区进行 5 或 10 次 SW 脉冲。使用小动物 PET/计算机断层扫描（CT）扫描仪，在基线、损伤后 3 小时和损伤后 3 天对大鼠进行 FDG 摄取扫描。在整个大脑、每个半球和四个皮质（运动、扣带、体感和 retrosplenial）中，将所有时间点的 FDG 摄取量作为大脑相对于心脏左心室（LV）中周围活性的活性比值（A/A）进行定量。半球和整体 FDG 摄取比的混合设计模型方差分析（ANOVA）显示扫描时间（p=0.038）和测量区域（p=6.12e-09）有显著影响。我们还观察到四个皮质区域的 FDG 摄取比在扫描时间（p=0.046）和测量区域（p=2.28e-09）方面有显著影响。在全球或局部测量中，SW 脉冲数（5 或 10）或 SW 位置（外侧或额前背区）均无显著影响。我们的数据表明，与基线测量相比，SW-TBI 在损伤后 3 小时导致大鼠大脑受冲击侧的代谢亢进。然而，损伤后 3 天 FDG 摄取的增加并不显著。需要进一步研究创伤后代谢变化，以更好地了解损伤的病理生理学。
