Department of Pharmaceutical analysis, The Fourth Military Medical University, Xi'an, Shaanxi, China.
Institute of Biomedical and Health Engineering, ShenZhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
Nanomedicine. 2018 Jan;14(1):109-121. doi: 10.1016/j.nano.2017.09.002. Epub 2017 Sep 18.
Chemo-photodynamic combination has been manifested great potential for synergistic cancer therapy. Moreover, the synergistic efficacy could be significantly enhanced by well-designed sequential release manner of photosensitizers (PSs). Here we propose a "big & small combo nanoparticles (NP)" system for double loading PSs methylene blue (MB) and single absorbing chemotherapeutics drug Gemcitabine hydrochloride (GM·HCl). The "grown-in" MB from NP show two-peak sequential release profile, significantly improve the absorbed chemotherapeutic efficacy of GM·HCl. The corresponding two-peak sequential release profile can be illustrated by related mathematics function. The sequential release property was clearly observed through morphological evolution of NPs both in water and cells by TEM. Furthermore, NP demonstrate well EPR effect and improved synergistic efficacy from in vitro and in vivo results. Thus, NP chemo-photodynamic system and the programmable sequential release mechanism provide a promising platform that ensures an enhanced synergistic chemo-photodynamic effect in cancer treatment.
化疗-光动力联合治疗已经显示出协同癌症治疗的巨大潜力。此外，通过精心设计的光敏剂（PSs）的顺序释放方式，可以显著增强协同疗效。在这里，我们提出了一种“大-小组合纳米颗粒（NP）”系统，用于双重负载 PS 亚甲蓝（MB）和单吸收化疗药物盐酸吉西他滨（GM·HCl）。NP 中“生长”的 MB 显示出双峰顺序释放曲线，显著提高了 GM·HCl 的吸收化疗效果。相关数学函数可以说明相应的双峰顺序释放曲线。通过 TEM 观察 NP 在水和细胞中的形态演变，清楚地观察到顺序释放特性。此外，NP 表现出良好的 EPR 效应，并从体外和体内结果提高了协同疗效。因此，NP 化疗-光动力系统和可编程顺序释放机制提供了一个有前途的平台，确保在癌症治疗中增强协同化疗-光动力效应。
