适用于真菌基因组大小估计的标准的验证。
Validation of standards suitable for genome size estimation of fungi.
作者信息
Talhinhas Pedro, Tavares Daniela, Ramos Ana Paula, Gonçalves Susana, Loureiro João
机构信息
LEAF-Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal.
CFE-Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, 3001-401 Coimbra, Portugal.
出版信息
J Microbiol Methods. 2017 Nov;142:76-78. doi: 10.1016/j.mimet.2017.09.012. Epub 2017 Sep 18.
Genome size information is fundamental to genome sequencing and may also uncover genomic aspects of evolution. Flow Cytometry, the preferred method for genome size estimation, requires suitable standards. Here we validate Inonotus hispidus, Colletotrichum acutatum and Cenococcum geophilum (41, 68 and 203Mbp), as standards for fungal genome size estimation.
基因组大小信息对于基因组测序至关重要，还可能揭示进化的基因组层面。流式细胞术是估计基因组大小的首选方法，需要合适的标准。在这里，我们验证了糙皮侧耳、尖孢炭疽菌和土生空团菌（分别为41、68和203兆碱基对）作为真菌基因组大小估计的标准。
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