Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center for Micro-Nano Biomedical Materials and Devices, Chongqing 400715, China.
Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center for Micro-Nano Biomedical Materials and Devices, Chongqing 400715, China.
J Colloid Interface Sci. 2018 Jan 1;509:384-394. doi: 10.1016/j.jcis.2017.09.027. Epub 2017 Sep 8.
Multifunctional nanoagents have become popular and valuable pharmaceuticals for effective cancer treatment. Moreover, there is an increasing tendency to develop therapeutic agents with excellent biocompatibility, high efficiency, specific targeting and combinatorial treatment effects. In this study, we proposed a facile technique to synthesize PEGylated (polyethylene glycol modified) magnetic Prussian blue (PB) nanoparticles with encapsulated doxorubicin (DOX), abbreviated as FeO@PB/PEG/DOX NPs, for combined targeted photothermal ablation and pH-triggered chemotherapy of tumour cells. The PEGylation of FeO@PB core-shell structure was achieved through a thin-film hydration process; DOX was loaded into the nanocapsule via hydrophobic interactions. An in vitro study indicated increased drug release under acidic conditions, mimicking mild acidic tumour microenvironments. Additionally, the nanocomposites exhibited superparamagnetism, contributing to an improved therapeutic effect guided by a localized magnetic field. Cytotoxicity studies demonstrated outstanding photothermal-chemotherapy combinatorial effects on HeLa cells, attributed to the targeted photothermic effect mediated by the pH-triggered cellular uptake of DOX. Specifically, the viability of HeLa cells decreased to 8.5% after treatment with the nanoagent (DOX=10μgmL) and near infrared irradiation, indicating an evident tumour inhibition effect in vitro. This study presented a nanoplatform for efficient and targeted cancer treatment, which may lead to the development of multifunctional nanodrug vehicles for cancer therapy.
多功能纳米制剂已成为癌症治疗中有效药物的热门选择。此外，人们越来越倾向于开发具有良好生物相容性、高效、靶向特异性和联合治疗效果的治疗剂。在这项研究中，我们提出了一种简便的技术，用于合成聚乙二醇化（聚乙二醇修饰）的磁性普鲁士蓝（PB）纳米粒子，并用阿霉素（DOX）包封，简称 FeO@PB/PEG/DOX NPs，用于肿瘤细胞的联合靶向光热消融和 pH 触发化疗。通过薄膜水合过程实现了 FeO@PB 核壳结构的 PEG 化；通过疏水相互作用将 DOX 载入纳米胶囊中。体外研究表明，在模拟轻度酸性肿瘤微环境的酸性条件下，药物释放增加。此外，该纳米复合材料具有超顺磁性，有助于在局部磁场引导下提高治疗效果。细胞毒性研究表明，HeLa 细胞对光热化疗联合具有出色的效果，这归因于 DOX 的 pH 触发细胞摄取介导的靶向光热效应。具体来说，在用纳米制剂（DOX=10μgmL）和近红外辐射处理后，HeLa 细胞的存活率降至 8.5%，表明体外具有明显的肿瘤抑制作用。本研究提出了一种高效、靶向癌症治疗的纳米平台，可能为癌症治疗的多功能纳米药物载体的发展提供了新的思路。
