Key Laboratory of Photochemical Biomaterials and Energy Storage Materials, Heilongjiang Province, College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin, 150025, P. R. China.
Dalton Trans. 2018 Feb 13;47(7):2435-2443. doi: 10.1039/c7dt04080f.
A facile methodology is presented to construct a multifunctional nanocomposite that integrates photothermal therapy and specific drug release into a single nanostructure. Firstly, magnetic FeO@polydopamine core-shell nanoparticles (FeO@PDA) were synthesized via a reversed-phase microemulsion approach. By varying the amount of DA, FeO@PDA with a particle size of 28-38 nm can be obtained. To further ensure the monodispersity, biocompatibility and specific uptake, PEG and lactobionic acid (LA) were grafted onto FeO@PDA (LA-FeO@PDA-PEG), whose fast photothermal conversion is derived by the combination of FeO and PDA with high near infrared (NIR) absorption. Then, doxorubicin hydrochloride (DOX) was adopted as the typical anticancer drug, which was loaded onto LA-FeO@PDA-PEG via electrostatic and π-π stacking interaction. The release kinetics investigation further demonstrated the acid/heat-triggered DOX release. HepG2 cells (hepatocellular cell line) were used as the target cancer cells, and the fast uptake was due to the nanoparticle size and abundant asialoglycoprotein receptors on HepG2 cells. Besides, an external magnetic field also can improve the uptake, especially when the magnet is placed at the bottom of the cell disk. The enhanced specific cytotoxicity toward HepG2 cells was also ascribed to the synergistic effect of chemo- and photothermal therapy. Based on the novel properties, the LA-FeO@PDA-PEG-DOX nanocomposite showed its potential application in hepatocyte therapy.
本文提出了一种简便的方法来构建多功能纳米复合材料，将光热治疗和特定药物释放整合到单个纳米结构中。首先，通过反相微乳液法合成了磁性 FeO@聚多巴胺核壳纳米粒子（FeO@PDA）。通过改变 DA 的用量，可以得到粒径为 28-38nm 的 FeO@PDA。为了进一步确保单分散性、生物相容性和特异性摄取，将 PEG 和乳糖酸（LA）接枝到 FeO@PDA 上（LA-FeO@PDA-PEG），其快速光热转换是由具有高近红外（NIR）吸收的 FeO 和 PDA 结合而来的。然后，盐酸阿霉素（DOX）被用作典型的抗癌药物，通过静电和π-π堆积相互作用将其负载到 LA-FeO@PDA-PEG 上。释放动力学研究进一步证明了酸/热触发的 DOX 释放。HepG2 细胞（肝细胞系）被用作靶癌细胞，由于纳米粒子的尺寸和 HepG2 细胞上丰富的去唾液酸糖蛋白受体，导致其快速摄取。此外，外加磁场也可以提高摄取效率，特别是当磁铁放在细胞盘底部时。对 HepG2 细胞的增强的特异性细胞毒性归因于化学和光热治疗的协同作用。基于这些新特性，LA-FeO@PDA-PEG-DOX 纳米复合材料显示出在肝细胞治疗中的潜在应用。
