Macroevolutionary shifts of function potentiate butterfly wing-pattern diversity.

Butterfly wing patterns provide a rich comparative framework to study how morphological complexity develops and evolves. Here we used CRISPR/Cas9 somatic mutagenesis to test a patterning role for , a signaling ligand gene previously identified as a hotspot of shape-tuning alleles involved in wing mimicry. We show that loss-of-function causes multiple modifications of pattern elements in seven nymphalid butterfly species. In three butterflies with a conserved wing-pattern arrangement, is necessary for the induction of stripe-like patterns known as symmetry systems and acquired a novel eyespot activator role specific to forewings. In two species, specifies the boundaries between melanic fields and the light-color patterns that they contour. In the passionvine butterfly , removal shows opposite effects on adjacent pattern elements, revealing a dual role across the wing field. Finally, acquired a divergent role in the patterning of interveinous patterns in the monarch, a basal nymphalid butterfly that lacks stripe-like symmetry systems. These results identify as an instructive signal for the prepatterning of a biological system of exuberant diversity and illustrate how shifts in the deployment and effects of a single developmental gene underlie morphological change.