Schubert M L, Makhlouf G M
Department of Medicine, Medical College of Virginia, Richmond 23298.
Am J Physiol. 1987 Dec;253(6 Pt 1):G721-5. doi: 10.1152/ajpgi.1987.253.6.G721.
A muscle-stripped mucosal sheet obtained from rat antrum was mounted in an Ussing chamber and used to examine the regulation of gastrin and somatostatin secretion by antral neurons. The neurons were activated pharmacologically with 1,1-dimethyl-4-phenylpiperazinium (DMPP) or electrically by field stimulation using aluminum foil electrodes layered over the edge of the mucosal sheet. Field stimulation caused an increase in gastrin and somatostatin secretion that was dependent on the strength of the stimulus (10-30 V). Field stimulation at 30 V, 10 Hz, 4 ms caused a sixfold increase in gastrin and a twofold increase in somatostatin secretion that were nearly abolished by tetrodotoxin (85-89% inhibition). Atropine partially inhibited the gastrin response (34 +/- 6%) but had no effect on the somatostatin response. DMPP caused lesser, though significant, increases in gastrin (166%) and somatostatin (83%) secretion that were nearly abolished by hexamethonium (84-91%) but were not significantly affected by atropine. The increase in somatostatin secretion caused by DMPP and field stimulation, as well as the resistance of gastrin and somatostatin secretion to atropine, was consistent with preferential activation of noncholinergic neurons at the stimulatory modalities used. The pattern and magnitude of gastrin and somatostatin response to pharmacological and electrical stimulation of antral neurons were similar to those previously observed in the vascularly perfused whole rat stomach.
从大鼠胃窦获取的肌肉剥离黏膜片被安装在尤斯灌流小室中，用于研究胃窦神经元对胃泌素和生长抑素分泌的调节。通过用1,1 - 二甲基 - 4 - 苯基哌嗪鎓（DMPP）进行药理学激活或使用置于黏膜片边缘的铝箔电极进行电场刺激来激活神经元。电场刺激导致胃泌素和生长抑素分泌增加，这取决于刺激强度（10 - 30V）。30V、10Hz、4ms的电场刺激使胃泌素分泌增加6倍，生长抑素分泌增加2倍，而河豚毒素几乎完全抑制了这种增加（抑制率为85 - 89%）。阿托品部分抑制胃泌素反应（34±6%），但对生长抑素反应无影响。DMPP使胃泌素（166%）和生长抑素（83%）分泌有较小但显著的增加，六甲铵几乎完全抑制了这种增加（84 - 91%），但阿托品对其无显著影响。DMPP和电场刺激引起的生长抑素分泌增加，以及胃泌素和生长抑素分泌对阿托品的抵抗，与所用刺激方式下非胆碱能神经元的优先激活一致。胃窦神经元受到药理学和电刺激时，胃泌素和生长抑素反应的模式和幅度与先前在血管灌流的整个大鼠胃中观察到的相似。
