Schubert M L, Saffouri B, Walsh J H, Makhlouf G M
Am J Physiol. 1985 Apr;248(4 Pt 1):G456-62. doi: 10.1152/ajpgi.1985.248.4.G456.
In vitro studies on the vascularly perfused rat stomach have shown that gastrin secretion is regulated by intramural cholinergic and noncholinergic neurons. We have postulated that bombesin (gastrin-releasing peptide), a known gastrin stimulant present in antral mucosal nerve fibers, is the most likely candidate for noncholinergic transmitter. Bombesin antiserum (final dilution, 1:150) but not control serum added to the vascular perfusate inhibited the gastrin response to 1,1-dimethyl-4-phenylpiperazinium by 59 +/- 17% (P less than 0.01) and to electrical field stimulation by 60 +/- 16% (P less than 0.01), and its effect was additive to that of 10(-7) M atropine (75-94%), thus accounting for the greater part of neurally induced gastrin secretion. The effects of atropine and bombesin antiserum on somatostatin secretion were consistent also with blockade of cholinergic and noncholinergic neurons, respectively. The results indicate that bombesin and acetylcholine are the main intramural neural regulators of gastrin and somatostatin secretion. Acetylcholine acts predominantly to decrease the paracrine secretion of somatostatin, thereby eliminating the continuous restraint of somatostatin on gastrin secretion and enabling bombesin to exert its potent stimulatory effect on gastrin secretion.
对血管灌注大鼠胃进行的体外研究表明，胃泌素分泌受壁内胆碱能和非胆碱能神经元调节。我们推测，蛙皮素（胃泌素释放肽）是存在于胃窦黏膜神经纤维中的一种已知胃泌素刺激物，是最有可能的非胆碱能递质候选物。加入血管灌流液中的蛙皮素抗血清（终浓度稀释至1:150）而非对照血清，使胃泌素对1,1 - 二甲基 - 4 - 苯基哌嗪的反应抑制了59±17%（P<0.01），对电场刺激的反应抑制了60±16%（P<0.01），其作用与10(-7)M阿托品的作用相加（75 - 94%），从而解释了神经诱导胃泌素分泌的大部分原因。阿托品和蛙皮素抗血清对生长抑素分泌的作用也分别与胆碱能和非胆碱能神经元的阻断作用一致。结果表明，蛙皮素和乙酰胆碱是胃泌素和生长抑素分泌的主要壁内神经调节因子。乙酰胆碱主要作用是减少生长抑素的旁分泌分泌，从而消除生长抑素对胃泌素分泌的持续抑制，使蛙皮素能够对胃泌素分泌发挥强大的刺激作用。
