Miao Ning, Zhang Lei, Li Maoping, Fan Liqiang, Mao Kangshan
MOE Key Laboratory of Bio-Resources and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, People's Republic of China.
Appl Plant Sci. 2017 Aug 24;5(8). doi: 10.3732/apps.1700010. eCollection 2017 Aug.
We developed transcriptome microsatellite markers (simple sequence repeats) for (Loranthaceae) to survey the genetic diversity and population structure of this species.
We used Illumina HiSeq data to reconstruct the transcriptome of . by de novo assembly and used the transcriptome to develop a set of simple sequence repeat markers. Overall, 40 primer pairs were designed and tested; 19 of them amplified successfully and demonstrated polymorphisms. Two loci that detected null alleles were eliminated, and the remaining 17, which were subjected to further analyses, yielded two to 21 alleles per locus.
The markers will serve as a basis for studies to assess the extent and pattern of distribution of genetic variation in . , and they may also be useful in conservation genetic, ecological, and evolutionary studies of the genus , a group of plant species of importance in Chinese traditional medicine.
我们为桑寄生科植物开发了转录组微卫星标记(简单序列重复),以调查该物种的遗传多样性和种群结构。
我们使用Illumina HiSeq数据通过从头组装重建了该植物的转录组,并利用转录组开发了一组简单序列重复标记。总体而言,设计并测试了40对引物;其中19对成功扩增并显示出多态性。剔除了两个检测到无效等位基因的位点,其余17个位点进行了进一步分析,每个位点产生2至21个等位基因。
这些标记将为评估该植物遗传变异的分布范围和模式的研究提供基础,它们也可能有助于对该属进行保护遗传学、生态学和进化研究,该属是中国传统医学中一组重要的植物物种。