Sato Miyako, Hasegawa Yoichi, Mishima Kentaro, Takata Katsuhiko
Institute of Wood Technology, Akita Prefectural University, 11-1 Kaieisaka, Noshiro, Akita 016-0876, Japan.
Forestry and Forest Products Research Institute, Forest Tree Breeding Center, 3809-1 Ishi, Juo, Hitachi, Ibraraki 319-1301, Japan.
Appl Plant Sci. 2015 Jan 30;3(2). doi: 10.3732/apps.1400101. eCollection 2015 Feb.
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Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed from Thujopsis dolabrata var. hondae (Cupressaceae) using Illumina sequencing to investigate the genetic diversity and population structure of the genus Thujopsis. •
Twenty-two primer pairs were developed from ESTs of T. dolabrata var. hondae. The primers amplified di- and trinucleotide repeat-containing sequences. Polymorphisms were assessed in 81 individuals from two T. dolabrata var. hondae populations and one T. dolabrata population. The number of alleles ranged from one to 17 per locus. The observed and expected heterozygosities ranged from 0.000 to 1.000 and from 0.000 to 0.926, respectively. •
These new EST-SSR markers will be useful in analyses of the genetic diversity and population structure of the genus Thujopsis.
利用Illumina测序技术从日本扁柏（柏科）中开发表达序列标签-简单序列重复（EST-SSR）标记，以研究日本扁柏属的遗传多样性和种群结构。
从日本扁柏的ESTs中开发了22对引物。这些引物扩增出含有二核苷酸和三核苷酸重复序列。对来自两个日本扁柏种群和一个日本扁柏种群的81个个体进行了多态性评估。每个位点的等位基因数从1到17不等。观察到的杂合度和预期杂合度分别从0.000到1.000和从0.000到0.926。
这些新的EST-SSR标记将有助于分析日本扁柏属的遗传多样性和种群结构。
