红厚壳（玉蕊科）EST-SSR标记的开发及在相关物种中的交叉扩增
Development of EST-SSR markers in Barringtonia racemosa (Lecythidaceae) and cross-amplification in related species.
作者信息
Xie Hongxian, Yuan Yang, Fang Xiaoting, Liu Ying, Yang Chao, Jin Jianhua, Tan Fengxiao, Huang Yelin
机构信息
Guangdong Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China.
Guangdong Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, Guangdong, People's Republic of China ; Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong, People's Republic of China.
出版信息
Appl Plant Sci. 2015 Dec 4;3(12). doi: 10.3732/apps.1500080. eCollection 2015 Dec.
PREMISE OF THE STUDY
Microsatellite markers were identified and characterized to study the genetic diversity and structure of Barringtonia racemosa (Lecythidaceae).
METHODS AND RESULTS
Based on the transcriptome data of B. racemosa, 30 primer pairs were initially designed and tested, of which 15 were successfully amplified and displayed clear polymorphisms across the 43 individuals from three distant populations tested in the study. The results showed that the number of alleles per locus ranged from two to seven and the expected heterozygosity and observed heterozygosity per locus varied from 0 to 0.772 and from 0 to 0.933, respectively.
CONCLUSIONS
The expressed sequence tag-simple sequence repeat (EST-SSR) markers described here will be useful for studying genetic diversity and structure of B. racemosa. Furthermore, all loci were successfully cross-amplified in B. asiatica and B. acutangula and will be of great value for genetic studies across this genus.
研究前提
鉴定并表征微卫星标记，以研究总状苹婆（玉蕊科）的遗传多样性和结构。
方法与结果
基于总状苹婆的转录组数据，最初设计并测试了30对引物，其中15对在该研究中测试的来自三个不同种群的43个个体中成功扩增并显示出清晰的多态性。结果表明，每个位点的等位基因数从2到7不等，每个位点的预期杂合度和观察到的杂合度分别在0到0.772和0到0.933之间变化。
结论
本文所述的表达序列标签-简单序列重复（EST-SSR）标记将有助于研究总状苹婆的遗传多样性和结构。此外，所有位点在海南苹婆和锐尖苹婆中均成功进行了交叉扩增，对该属的遗传研究具有重要价值。
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