鹅掌楸（木兰科）微卫星位点的鉴定与特征分析
Identification and characterization of microsatellite loci in the tuliptree, (Magnoliaceae).
作者信息
Gutiérrez-Ozuna Ricardo, Hamilton Matthew B
机构信息
Department of Biology, Georgetown University, 37th and O Streets NW, Washington, D.C. 20057 USA.
出版信息
Appl Plant Sci. 2017 Aug 24;5(8). doi: 10.3732/apps.1700032. eCollection 2017 Aug.
PREMISE OF THE STUDY
Twenty-three polymorphic microsatellite loci (simple sequence repeats) were identified and characterized for (Magnoliaceae), a species native to eastern North America, to investigate its genetic diversity, population structure, and mating system.
METHODS AND RESULTS
Using Illumina HiSeq paired-end reads from genomic DNA, searches for repeat motifs identified approximately 280,000 potentially amplifiable loci. Of 77 loci tested, 51 amplified consistently. When genotyped using 30 to 52 total adult trees from three old-growth populations in Maryland, Virginia, and New Jersey, USA, 23 loci were polymorphic. These loci exhibited four to 13 alleles, and observed and expected heterozygosities ranged from 0.233 to 0.865 and 0.272 to 0.876, respectively.
CONCLUSIONS
The microsatellite marker loci presented here will be valuable in population genetic studies of because they do not suffer from ascertainment bias and show high polymorphism.
研究前提
为了研究北美木兰（Magnoliaceae）的遗传多样性、种群结构和交配系统，我们鉴定并表征了23个多态性微卫星位点（简单序列重复），该物种原产于北美东部。
方法与结果
利用来自基因组DNA的Illumina HiSeq双端读数，对重复基序的搜索鉴定出约280,000个潜在可扩增位点。在测试的77个位点中，51个位点能持续扩增。当对来自美国马里兰州、弗吉尼亚州和新泽西州三个老龄种群的30至52棵成年树木进行基因分型时，23个位点具有多态性。这些位点表现出4至13个等位基因，观察到的杂合度和预期杂合度分别为0.233至0.865和0.272至0.876。
结论
本文所呈现的微卫星标记位点在北美木兰的种群遗传学研究中将具有重要价值，因为它们不存在鉴定偏差且具有高度多态性。
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