鉴定 39 个新型 Liriodendron tulipifera 表达序列标签简单重复序列标记并在 L. chinense （木兰科）中进行跨物种扩增。

Characterization of 39 novel EST-SSR markers for Liriodendron tulipifera and cross-species amplification in L. chinense (Magnoliaceae).

机构信息

Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, Hubei, People's Republic of China.

出版信息

Am J Bot. 2012 Nov;99(11):e460-4. doi: 10.3732/ajb.1200154. Epub 2012 Oct 29.

DOI:10.3732/ajb.1200154

PMID:23108462

Abstract

PREMISE OF THE STUDY

A novel set of simple sequence repeat (SSR) markers were developed and characterized from the expressed sequence tag (EST) database of Liriodendron tulipifera for application in population genetic studies of Liriodendron.

METHODS AND RESULTS

Thirty-nine polymorphic EST-SSR loci were identified among 27 individuals sampled from a cultivated population of L. tulipifera. The number of alleles per locus ranged from three to 18. The average observed heterozygosity and expected heterozygosity were 0.684 and 0.778, respectively. Of the 39 loci, 32 showed interspecific transferability and polymorphism in a related species, L. chinense. The number of alleles per locus ranged from two to 11, and the average observed heterozygosity and expected heterozygosity were 0.475 and 0.736, respectively.

CONCLUSIONS

The developed EST-SSR markers will be useful for investigating adaptive genetic differentiation in Liriodendron.

摘要

研究前提

从鹅掌楸（Liriodendron tulipifera）的表达序列标签（EST）数据库中开发和鉴定了一组新的简单重复序列（SSR）标记，用于鹅掌楸种群遗传研究。

方法和结果

从鹅掌楸人工栽培群体中随机抽取的 27 个个体中鉴定出 39 个多态性 EST-SSR 位点。每个位点的等位基因数范围为 3 至 18。平均观测杂合度和期望杂合度分别为 0.684 和 0.778。在 39 个位点中，有 32 个在相关物种杂种鹅掌楸（L. chinense）中表现出种间转移和多态性。每个位点的等位基因数范围为 2 至 11，平均观测杂合度和期望杂合度分别为 0.475 和 0.736。