Super-Resolution High Content Screening and Analysis.

Revealing the subcellular phenotypes at the molecular scale is critical to understand the mechanisms by which the cells function and respond to chemical treatments. Super-resolution microscopy and robust analysis tools enabled biologists to reveal and quantify phenotypes at unprecedented resolution. Developing automated imaging analysis solutions for super-resolution imaging will make high-content-screening (HCS) applicable for super-resolution microscopy, which will give access to new complex information. Here, I provide an instant automated analysis procedure for analyzing super-resolution images via CellProfiler ( www.cellprofiler.org ) platform.