School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, P.R. China.
School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, 510006, P.R. China.
Phytochem Anal. 2018 Mar;29(2):168-179. doi: 10.1002/pca.2725. Epub 2017 Sep 18.
Ilex pubescens Hook et Arn mainly contains triterpenoids that possess antithrombotic, anti-inflammatory and analgesic effects. Quantitative and qualitative analyses of the triterpenoids in I. pubescens can be useful for determining the authenticity and quality of raw materials and guiding its clinical preparation.
To establish a method for rapid and comprehensive analysis of triterpenoids in I. pubescens using ultra-high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight-mass spectrometry (UPLC-ESI-QTOF-MS), which will also be applied to evaluate the contents of nine triterpenoids among root, root heartwood and root bark of I. pubescens to judge the value of the root bark to avoid wastage.
UPLC-ESI-QTOF-MS data from the extracts of I. pubescens in negative mode were analysed using Peakview and Masterview software that provided molecular weight, mass errors, isotope pattern fit and MS/MS fragments for the identification of triterpenoids. The quantification of nine investigated compounds of I. pubescens was accomplished using MultiQuant software.
A total of 33 triterpenoids, five phenolic acids, two lignans and a flavonol were characterised in only 14 min. The total content of the nine compounds in the root bark was generally slightly higher than that of the root and root heartwood, which has not been reported before.
The developed UPLC-ESI-QTOF-MS method was proven to be rapid and comprehensive for simultaneous qualitative and quantitative analyses of the characteristic triterpenoids in I. pubescens. The results may provide a basis for holistic quality control and metabolic studies of I. pubescens, as well as serve as a reference for the analysis of other Ilex plants. Copyright © 2017 John Wiley & Sons, Ltd.
绒毛八角莲主要含有三萜类化合物，具有抗血栓、抗炎和镇痛作用。绒毛八角莲中三萜类化合物的定量和定性分析有助于确定原料的真实性和质量，并指导其临床制备。
建立一种超高效液相色谱-电喷雾离子化-四极杆飞行时间质谱联用（UPLC-ESI-QTOF-MS）快速全面分析绒毛八角莲中三萜类化合物的方法，并应用于评价绒毛八角莲的根、根心材和根皮中 9 种三萜类化合物的含量，以判断根皮的价值，避免浪费。
采用 Peakview 和 Masterview 软件对绒毛八角莲提取物的负模式 UPLC-ESI-QTOF-MS 数据进行分析，提供三萜类化合物的分子量、质量误差、同位素模式拟合和 MS/MS 碎片，用于鉴定三萜类化合物。采用 MultiQuant 软件对 9 种绒毛八角莲化合物进行定量分析。
在 14 分钟内共鉴定了 33 种三萜类化合物、5 种酚酸、2 种木脂素和 1 种黄酮醇。根皮中 9 种化合物的总含量普遍略高于根和根心材，这是以前没有报道过的。
所建立的 UPLC-ESI-QTOF-MS 方法快速、全面，可同时对绒毛八角莲中特征性三萜类化合物进行定性和定量分析。研究结果可为绒毛八角莲的整体质量控制和代谢研究提供依据，也可为其他八角莲属植物的分析提供参考。版权所有©2017 约翰威立父子公司
