[酸碱预处理后酶解对丹参残渣中丹参酮提取的影响]
[Effect of enzymolysis after acid and alkali pretreatment on extraction of tanshinones from Salviae Miltiorrhizae Radix et Rhizoma residues].
作者信息
Dai Xin-Xin, Shen Fei, Su Shu-Lan, Zhang Sen, Guo Sheng, Jiang Shu, Qian Da-Wei, Duan Jin-Ao
机构信息
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, State Administration of Traditional Chinese Medicine, Traditional Chinese Medicine Resource Recycling, Nanjing University of Chinese Medicine, Nanjing 210023, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2016 Sep;41(18):3355-3360. doi: 10.4268/cjcmm20161808.
Salviae Miltiorrhizae Radix et Rhizoma residues were pre-treated with acid and alkali, degraded by using cellulose, and the effects of different processing methods on the extraction rate of tanshinones were compared to provide scientific basis for development and utilization of tanshinones from Salviae Miltiorrhizae Radix et Rhizoma residues. The results showed that in the Salviae Miltiorrhizae Radix et Rhizoma residues without pre-treatment, enzymatic hydrolysis time of 4.5 d could make most of the cellulose degraded when the concentration of substrate enzyme concentration was 6 U•mL-1, and the highest glucose concentration was 59.74 mg•g⁻¹. It was found that the best effect was achieved after alkali pre-treatment-cellulose C degradation among the different pre-treatment methods, and the glucose content reached 119.50 mg•g⁻¹, followed by the same concentration of acid pre-treatment-cellulose C degradation. The extraction amount of tanshinone ⅡA was increased by 82.54% after enzyme degradation, with a mass fraction of 2.451 mg•g⁻¹; extraction amount of tanshinone I was increased by 81.82% after enzyme degradation, with a mass fraction of 2.373 mg•g⁻¹; extraction amount of cryptotanshinone was increased by 64.4% after enzyme degradation, with a mass fraction of 1.080 mg•g⁻¹; extraction amount of dihydrotanshinone I was increased by 61.3% after enzyme degradation, with a mass fraction of 0.601 2 mg•g⁻¹. Acid and alkali pre-treatment combined with cellulose degradation could effectively improve the extraction rate of tanshinones from Salviae Miltiorrhizae Radix et Rhizoma residues. This method is operable and practical, and it is beneficial for improving the utilization efficiency of tanshinones (resource based chemicals) from Salviae Miltiorrhizae Radix et Rhizoma residues.
对丹参残渣进行酸碱预处理，利用纤维素酶进行降解，比较不同处理方法对丹参酮提取率的影响，为丹参残渣中丹参酮的开发利用提供科学依据。结果表明，未经预处理的丹参残渣，在底物酶浓度为6 U•mL-1时，酶解4.5 d可使大部分纤维素降解，葡萄糖最高浓度为59.74 mg•g⁻¹。在不同预处理方法中，碱预处理-纤维素C降解效果最佳，葡萄糖含量达119.50 mg•g⁻¹，其次为同浓度酸预处理-纤维素C降解。酶解后丹参酮ⅡA提取量提高82.54%，质量分数为2.451 mg•g⁻¹；丹参酮Ⅰ提取量提高81.82%，质量分数为2.373 mg•g⁻¹；隐丹参酮提取量提高64.4%，质量分数为1.080 mg•g⁻¹；二氢丹参酮Ⅰ提取量提高61.3%，质量分数为0.601 2 mg•g⁻¹。酸碱预处理结合纤维素酶降解可有效提高丹参残渣中丹参酮的提取率。该方法具有可操作性和实用性，有利于提高丹参残渣中丹参酮（资源型化学品）的利用效率。
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