Mirjani Roohollah, Shahverdi Ahmad-Reza, Iranshahi Mehrdad, Amin Gholamreza, Shafiee Abbas
a Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran.
b Department of Pharmacognosy, Faculty of Pharmacy, Mashad University of Medical Sciences, Mashad, Iran.
Pharm Biol. 2005;43(4):293-295. doi: 10.1080/13880200590951658.
In this investigation, the antifungal activities of chloroform and water extracts of Ferula persica. var. persica. Willd. roots were studied using conventional disk diffusion method. The chloroform extract showed antifungal activity at concentrations tested. Bioassay-guided fractionation study of the chloroform extract by preparative thin-layer chromatography (PTLC) detected two antifungal fractions that were effective against some fungi. Using conventional spectroscopy methods, the active fractions were identified as t-butyl 3-[(1-methylpropyl)dithio]-2-propenyl malonate (persicasulfide A) (1) and t.-butyl 3-[(1-methylthiopropyl)dithio]-2-propenyl malonate (persicasulfide B) (2), recently isolated from Ferula persica. var. latisecta.. These compounds showed the most potent antifungal activity with MICs of ≤ 62.5 µg/ml against filamentous fungi.
在本研究中，采用传统的纸片扩散法研究了波斯阿魏（Ferula persica. var. persica. Willd.）根的氯仿提取物和水提取物的抗真菌活性。氯仿提取物在测试浓度下表现出抗真菌活性。通过制备薄层色谱法（PTLC）对氯仿提取物进行生物测定导向的分级分离研究，检测到两个对某些真菌有效的抗真菌级分。使用传统光谱方法，活性级分被鉴定为3-[(1-甲基丙基)二硫代]-2-丙烯基丙二酸叔丁酯（波斯硫化物A）（1）和3-[(1-甲基硫代丙基)二硫代]-2-丙烯基丙二酸叔丁酯（波斯硫化物B）（2），它们最近从宽裂叶波斯阿魏（Ferula persica. var. latisecta.）中分离得到。这些化合物对丝状真菌表现出最有效的抗真菌活性，最低抑菌浓度（MIC）≤62.5μg/ml。
