He Bin, Wei Wen, Liu Ji, Xu Yundan, Zhao Gang
Department of Medical Biology, School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China.
Department of Traditional Chinese Medicine, The Third People's Hospital of Hubei (Zhongshan Hospital of Hubei), Wuhan, Hubei 430033, P.R. China.
Oncol Lett. 2017 Sep;14(3):3387-3394. doi: 10.3892/ol.2017.6627. Epub 2017 Jul 20.
Curcumin is an anticancer compound that exerts anti-proliferative and apoptotic effects via multiple molecular targets. The purpose of the present study was to investigate the anticancer effects of curcumin in combination with 5-fluorouracil plus cisplatin (FP) on the MGC-803 human gastric cancer cell line. Following treatment with curcumin and/or FP for 24, 48 and 72 h, cell viability, cell cycle progression and the apoptosis rate were evaluated using an MTT assay, flow cytometry and dual acridine orange/ethidium bromide staining, respectively. In addition, colony formation, Transwell migration and caspase-3/caspase-8 activity assays were performed. The expression of the apoptosis regulator B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax) were detected by western blotting analysis. Following treatment with curcumin and/or FP, cell viability, colony formation and cell migration were significantly reduced compared with the untreated control group. The rate of apoptosis, caspase-3/caspase-8 activity and the expression of Bax were significantly increased, whereas Bcl-2 expression was significantly reduced following treatment with curcumin and/or FP, compared with the untreated control group. The efficacy of curcumin combined with low-dose FP was significantly increased, compared with that of curcumin combined with high-dose FP (P<0.05). Therefore, curcumin may enhance the anticancer effects of FP chemotherapy in MGC-803 cells through the promotion of apoptosis via the caspase-3/caspase-8, Bcl-2 and Bax signaling pathways. These results suggest that curcumin may serve as a synergistic drug with chemotherapy regimen FP for the treatment of gastric cancer.
姜黄素是一种抗癌化合物，可通过多个分子靶点发挥抗增殖和凋亡作用。本研究的目的是探讨姜黄素联合5-氟尿嘧啶加顺铂（FP）对MGC-803人胃癌细胞系的抗癌作用。在用姜黄素和/或FP处理24、48和72小时后，分别使用MTT法、流式细胞术和双吖啶橙/溴化乙锭染色评估细胞活力、细胞周期进程和凋亡率。此外，还进行了集落形成、Transwell迁移和caspase-3/caspase-8活性测定。通过蛋白质印迹分析检测凋亡调节因子B细胞淋巴瘤-2（Bcl-2）和Bcl-2相关X蛋白（Bax）的表达。与未处理的对照组相比，用姜黄素和/或FP处理后，细胞活力、集落形成和细胞迁移显著降低。与未处理的对照组相比，用姜黄素和/或FP处理后，凋亡率、caspase-3/caspase-8活性和Bax的表达显著增加，而Bcl-2的表达显著降低。与姜黄素联合高剂量FP相比，姜黄素联合低剂量FP的疗效显著提高（P<0.05）。因此，姜黄素可能通过caspase-3/caspase-8、Bcl-2和Bax信号通路促进凋亡，从而增强FP化疗对MGC-803细胞的抗癌作用。这些结果表明，姜黄素可能作为一种与化疗方案FP协同的药物用于治疗胃癌。
