Ferreira Lilian Dos Anjos Oliveira, Oliveira Mariana Maciel de, Faleiro Flávia Lais, Scariot Débora Botura, Boeing Joana Schuelter, Visentainer Jesuí Vergílio, Romagnolo Mariza Barion, Nakamura Celso Vataru, Truiti Maria da Conceição Torrado
a Programa de Pós-Graduação em Ciências Farmacêuticas , Universidade Estadual de Maringá , Maringá , Brazil.
b Departamento de Farmácia , Universidade Estadual de Maringá , Maringá , Brazil.
Nat Prod Res. 2018 Dec;32(23):2825-2828. doi: 10.1080/14786419.2017.1378214. Epub 2017 Sep 19.
The effects on the viability of promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and citotoxicity in J774.A1 macrophages of ethanolic extract, fractions and isolated compounds that were obtained from the leaves of Nectandra cuspidata were determined. The total phenolics content (TP) and antioxidant activity were assessed using different assays. The ethyl acetate fraction (EAF) had the best antiproliferative activity (amastigote IC: 4.42 ± 0.60 μg/mL, promastigote IC: 33.33 ± 0.78) and presented no cytotoxicity at the highest concentration tested (1000 μg/mL). The EAF also had the greatest antioxidant capacity, which was comparable to butylated hydroxytoluene and quercetin (DPPH IC 6.54 ± 0.10 μg/mL, ABTS 4.75 ± 0.11 mmol ET/g; FRAP 2.37 ± 0.03 mmol ET/g; ORAC = 33.52 ± 1.99 mmol ET/g; TP 387.11 ± 0.25 mg GAE/g). Vitexin, epicatechin and isovitexin isolated from EAF contribute to both activities.
测定了从尖叶楠木叶中获得的乙醇提取物、馏分和分离化合物对亚马逊利什曼原虫（Leishmania）前鞭毛体和无鞭毛体形式的活力影响以及对J774.A1巨噬细胞的细胞毒性。使用不同的分析方法评估了总酚含量（TP）和抗氧化活性。乙酸乙酯馏分（EAF）具有最佳的抗增殖活性（无鞭毛体IC：4.42±0.60μg/mL，前鞭毛体IC：33.33±0.78），并且在测试的最高浓度（1000μg/mL）下没有细胞毒性。EAF还具有最大的抗氧化能力，与丁基羟基甲苯和槲皮素相当（DPPH IC 6.54±0.10μg/mL，ABTS 4.75±0.11mmol ET/g；FRAP 2.37±0.03mmol ET/g；ORAC = 33.52±1.99mmol ET/g；TP 387.11±0.25mg GAE/g）。从EAF中分离出的牡荆素、表儿茶素和异牡荆素对这两种活性都有贡献。
