Departamento de Farmácia, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
Departamento de Química, Universidade Estadual de Maringá, Maringá, Paraná, Brazil.
J Photochem Photobiol B. 2018 Apr;181:127-133. doi: 10.1016/j.jphotobiol.2018.03.008. Epub 2018 Mar 9.
The total phenolic content (TP) and antioxidant activity of the ethanolic extract and fractions that were obtained from the leaves of Nectandra hihua were assessed using different methods. The ethanolic extract (EE) and ethyl acetate fraction (EAF) had the best antioxidant capacity, which was comparable to butylated hydroxytoluene and quercetin (ABTS 2.55 ± 0.06, 3.54 ± 0.03 mmol TE/g; DPPH IC 10.27 ± 0.05, 9.88 ± 0.02 μg/mL; FRAP 2.17 ± 0.08, 2.38 ± 0.04 mmol TE/g; ORAC 5.16 ± 0.08, 5.35 ± 0.07 mmol TE/g; TP 568.05 ± 18.15, 397.20 ± 17.88 mg GAE/g, respectively). The cytoprotective effect, reactive oxygen species (ROS) and lipid peroxidation inhibitions on L929 fibroblasts irradiated with UVB (600 mJ/cm) in pre- and post-treatments with EE and EAF were determined. These plant materials demonstrated high ROS scavenging activity and lipid peroxidation inhibition on L929 fibroblasts in both treatments, especially with pre-treatment (EE 38.47 ± 1.95% and EAF 40.20 ± 4.5% inhibition of ROS production, and EE 39.03 ± 3.33% and EAF 41.67 ± 7.6% of lipid peroxidation inhibition), indicating the best cytoprotection with pre-treatment (13.52 ± 1.66% and 13.34 ± 2.61% increases in cell viability). The antioxidant flavonoids quercitrin, avicularin, juglalin, afzelin and astragalin were isolated from EAF. The results obtained indicate that EE and EAF present photodamage attenuating potential against UVB-induced oxidative stress and can be useful as a starting point for developing dermatological products to prevent oxidative skin damage.
采用不同方法评估了来自 Nectandra hihua 叶的乙醇提取物和馏分的总酚含量 (TP) 和抗氧化活性。乙醇提取物 (EE) 和乙酸乙酯馏分 (EAF) 具有最佳的抗氧化能力，与丁基羟基甲苯和槲皮素相当（ABTS 2.55±0.06，3.54±0.03 mmol TE/g；DPPH IC 10.27±0.05，9.88±0.02 μg/mL；FRAP 2.17±0.08，2.38±0.04 mmol TE/g；ORAC 5.16±0.08，5.35±0.07 mmol TE/g；TP 分别为 568.05±18.15、397.20±17.88 mg GAE/g）。在 UVB（600 mJ/cm）辐照前后用 EE 和 EAF 对 L929 成纤维细胞进行预处理和后处理，测定其细胞保护作用、对 L929 成纤维细胞的活性氧 (ROS) 和脂质过氧化抑制作用。这些植物材料在两种处理方式下均表现出对 L929 成纤维细胞的高 ROS 清除活性和脂质过氧化抑制作用，尤其是预处理时（EE 对 ROS 产生的抑制率为 38.47±1.95%，EAF 为 40.20±4.5%；EE 对脂质过氧化的抑制率为 39.03±3.33%，EAF 为 41.67±7.6%），表明预处理时的细胞保护作用最佳（细胞活力分别增加 13.52±1.66%和 13.34±2.61%）。从 EAF 中分离出抗氧化黄酮槲皮素、芦丁、胡桃酮、杨梅素和黄芪素。结果表明，EE 和 EAF 具有减轻 UVB 诱导的氧化应激的光损伤潜力，可作为开发预防皮肤氧化损伤的皮肤科产品的起点。
