Endocrine pancreas of Testudo graeca L. (Chelonia) in summer and winter: an immunocytochemical and ultrastructural study.

Insulin-, glucagon-, somatostatin-, and PP-immunoreactive cells were identified immunocytochemically using antisera raised against mammalian hormones in the pancreas of Testudo graeca in both winter and summer. The endocrine cells were present throughout the gland, forming scarce islets except in the splenic region. The insulin cell islets were larger and more numerous in the splenic region than in the duodenal one. Winter glucagon-immunoreactive cells were found mainly in isolation while the summer ones occurred in groups which showed no immunoreactive central area; in both seasons these cells were more numerous in the splenic region than in the duodenal one. Somatostatin-immunoreactive cells were found isolated or grouped together more frequently in the splenic region in the summer specimens. No PP-immunoreactive cells were found in the splenic region, although they were numerous and isolated in the duodenal zone. Four cell types (B, A, D, and PP cells) were ultrastructurally characterized by the shape, size, and electron density of their respective secretory granules. Certain ultrastructural differences were detected in the summer and winter endocrine pancreatic cells. In summer specimens a fifth cellular type was observed. The presence of B, D, and PP cells among the epithelial pancreatic duct cells may confirm the comparatively primitive organization of the T. graeca endocrine pancreas.