对胸腺肌样细胞表达的Thy-1抗原进行的半定量免疫组织化学研究。

Semi-quantitative immunohistochemical studies on Thy-1 antigen expressed by thymic myoid cells.

作者信息

Kikuchi A, Kamo I, Fujisawa K, Nonaka I

机构信息

National Institute of Neuroscience, NCNP, Tokyo, Japan.

出版信息

J Neuroimmunol. 1988 Feb;17(3):217-28. doi: 10.1016/0165-5728(88)90070-7.

DOI:10.1016/0165-5728(88)90070-7

PMID:2892857

Abstract

Thy-1 antigen expression in the rat thymic myoid cell line R615B2 (Thy-1(+] and R613Ad (Thy-1(-] was studied with a semi-quantitative immunohistochemical assay without disrupting the cell cultures. With this assay, the quantity of Thy-1 antigen on R615B2 cells was detected separately in the cytoplasm and on the cell surface by the use of appropriate fixatives such as chilled ethanol and methanol + 0.3% H2O2. Extracellular Thy-1 antigen was also found in the culture supernatant of R615B2 cells. More than half of extracellular Thy-1 antigen remained in the supernatant even after 100,000 X g centrifugation. No form of Thy-1 antigen was detected at significant levels in R613Ad cells.

摘要

采用半定量免疫组织化学分析方法，在不破坏细胞培养的情况下，对大鼠胸腺肌样细胞系R615B2（Thy-1阳性）和R613Ad（Thy-1阴性）中的Thy-1抗原表达进行了研究。通过该分析方法，使用适当的固定剂（如冷乙醇和甲醇+0.3%过氧化氢），分别在细胞质和细胞表面检测R615B2细胞上Thy-1抗原的量。在R615B2细胞的培养上清液中也发现了细胞外Thy-1抗原。即使在100,000×g离心后，超过一半的细胞外Thy-1抗原仍保留在上清液中。在R613Ad细胞中未检测到显著水平的任何形式的Thy-1抗原。