Paul L C, Rennke H G, Milford E L, Carpenter C B
Kidney Int. 1984 May;25(5):771-7. doi: 10.1038/ki.1984.89.
The antigenic composition of the rat kidney was studied by indirect immunofluorescence and immunoelectron microscopy using monoclonal antibodies against Thy-1.1 (OX7 and ER Ly 1), Thy-1.2 (30-H12), and W3/13 as well as rabbit antisera against rat brain or thymic blasts. The anti-Thy-1.1 reagents caused pronounced staining of the glomerular mesangium as well as a weak stain around some of the medullary collecting ducts and/or vessels. The rabbit antisera contained additional antibodies that bound to renal basement membranes and smooth muscle cells. This was confirmed by blocking and absorption experiments. The presence of Thy-1.1 in glomeruli was further demonstrated by in vivo perfusion studies with OX7 and rabbit anti-thymic blast serum. Immunoelectron microscopy of isolated glomeruli showed binding of OX7 antibodies to mesangial cells and the mesangial matrix. It is concluded that in rat kidneys the Thy-1.1 antigen is expressed on glomerular mesangial cells.
利用抗Thy-1.1（OX7和ER Ly 1）、Thy-1.2（30-H12）和W3/13的单克隆抗体以及抗大鼠脑或胸腺母细胞的兔抗血清，通过间接免疫荧光和免疫电子显微镜研究了大鼠肾脏的抗原组成。抗Thy-1.1试剂使肾小球系膜明显染色，并且在一些髓质集合管和/或血管周围产生弱阳性染色。兔抗血清含有与肾基底膜和平滑肌细胞结合的其他抗体。这通过阻断和吸收实验得到证实。用OX7和兔抗胸腺母细胞血清进行的体内灌注研究进一步证明了肾小球中Thy-1.1的存在。分离肾小球的免疫电子显微镜显示OX7抗体与系膜细胞和系膜基质结合。得出的结论是，在大鼠肾脏中，Thy-1.1抗原在肾小球系膜细胞上表达。
