Kottaridi Christine, Spathis Aris, Margari Niki, Koureas Nikolaos, Terzakis Emmanouil, Chrelias Charalampos, Pappas Asimakis, Bilirakis Evripidis, Pouliakis Abraham, Panayiotides Ioannis J, Karakitsos Petros
Department of Cytopathology, University General Hospital "ATTIKON", School of Medicine, National and Kapodistrian University of Athens, Athens 12464, Greece.
Department of Obstetrics and Gynaecology, "Saint Savvas" Anticancer Hospital, Athens 11522, Greece.
J Cancer. 2017 Aug 22;8(14):2699-2703. doi: 10.7150/jca.19947. eCollection 2017.
miRNAs have an important role as their deregulation is linked to endometrial cancer. : A custom miScript® miRNA PCR Array was used to investigate for the first time the expression of eight miRNAs in forty-nine histologically confirmed Liquid Based cytology endometrial samples. The expression profile of the same miRNAs was also examined in sixty formalin-fixed tissue samples. Expression of seven miRNAs was significantly higher in malignant samples with three of them (mir-182, mir-141 and mir-205) performing optimally. These results suggest the potential use of this non-invasive method of sampling for miRNA expression studies. Furthermore miRNA overexpression could serve as an ancillary or reflex test for optimal identification of malignant samples especially in morphologically inadequate samples.
微小RNA（miRNAs）具有重要作用，因为它们的失调与子宫内膜癌有关。首次使用定制的miScript® miRNA PCR阵列研究了49例经组织学证实的液基细胞学子宫内膜样本中8种miRNAs的表达情况。还在60例福尔马林固定的组织样本中检测了相同miRNAs的表达谱。在恶性样本中，7种miRNAs的表达显著更高，其中3种（mir-182、mir-141和mir-205）表现最佳。这些结果表明这种非侵入性采样方法在miRNA表达研究中的潜在用途。此外，miRNA过表达可作为辅助或补充检测，用于最佳地识别恶性样本，尤其是在形态学上不充分的样本中。
