Karami Sahar, Roayaei Mohammad, Zahedi Elnaz, Bahmani Mahmoud, Mahmoodnia Leila, Hamzavi Hosna, Rafieian-Kopaei Mahmoud
Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahwaz, Iran.
Department of Pharmacology, Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Int J Pharm Investig. 2017 Apr-Jun;7(2):77-81. doi: 10.4103/jphi.JPHI_9_17.
The is a genus of lactic acid bacteria which are regularly rod-shaped, nonspore, Gram-positive, heterogeneous, and are found in a wide range of inhabitants such as dairy products, plants, and gastrointestinal tract. A variety of antimicrobial compounds and molecules such as bacteriocin are produced by these useful bacteria to inhibit the growth of pathogenic microbes in the food products. This paper aims to examine the isolation of from local dairies as well as to determine their inhibition effect against a number of pathogens, such as two fungi: and .
Twelve isolates from several local dairies. After initial dilution (10-10) and culture on the setting, de Man, Rogosa and Sharpe-agar, the isolates were recognized and separated by phenotypic characteristics and biochemical; then their antifungal effect was examined by two methods.
Having separated eight isolates, about 70% of the isolates have shown the inhabiting areas of antifungus on the agar-based setting, but two species alimentarius and delbrueckii have indicated a significant antifungal effect against and . Except bacteriocin, lactic acid, and acetic acid, the inhibitor substance is produced by these bacteria.
Given the vitality of in human health, recognition and isolation of the species producing compound in antagonist to the pathogens existing in the food products can be a helpful and effective step toward maintaining the valuable native and using them in the dairy industries.
该属乳酸菌通常呈杆状，无芽孢，革兰氏阳性，具有异质性，存在于多种宿主中，如乳制品、植物和胃肠道。这些有益细菌会产生多种抗菌化合物和分子，如细菌素，以抑制食品中致病微生物的生长。本文旨在从当地乳制品中分离该属细菌，并确定它们对多种病原体的抑制作用，如两种真菌：和。
从当地几家乳制品厂分离出12株该属细菌。在进行初步稀释（10 - 10）并在德氏、罗氏和夏普氏琼脂培养基上培养后，通过表型特征和生化特性对分离株进行识别和分离；然后通过两种方法检测它们的抗真菌效果。
分离出8株该属细菌，约70%的分离株在琼脂培养基上显示出抗真菌活性区域，但嗜食亚种和德氏亚种对和显示出显著的抗真菌效果。除细菌素、乳酸和乙酸外，这些细菌还产生抑制物质。
鉴于该属细菌对人类健康的重要性，识别和分离能够产生对抗食品中病原体的化合物的菌株，对于保护珍贵的本土该属细菌并将其应用于乳制品行业而言，可能是一个有益且有效的步骤。
