Moura Ana Rafaela Silva Simões, Kretz Cécilia Batmalle, Ferreira Italo Eustáquio, Nunes Amélia Maria Pithon Borges, de Moraes José Cássio, Reis Mitermayer Galvão, McBride Alan John Alexander, Wang Xin, Campos Leila Carvalho
Laboratório de Patologia e Biologia Molecular, Instituto Gonçalo Moniz, FIOCRUZ-BA, Salvador, Bahia, Brazil.
Meningitis and Vaccine Preventable Diseases Branch, Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, United States of America.
PLoS One. 2017 Sep 20;12(9):e0185038. doi: 10.1371/journal.pone.0185038. eCollection 2017.
Characterization of meningococci isolated from the pharynx is essential towards understanding the dynamics of meningococcal carriage and disease. Meningococcal isolates, collected from adolescents resident in Salvador, Brazil during 2014, were characterized by multilocus sequence typing, genotyping or whole-genome sequencing. Most were nongroupable (61.0%), followed by genogroups B (11.9%) and Y (8.5%). We identified 34 different sequence types (STs), eight were new STs, distributed among 14 clonal complexes (cc), cc1136 represented 20.3% of the nongroupable isolates. The porA and fetA genotypes included P1.18,25-37 (11.9%), P1.18-1,3 (10.2%); F5-5 (23.7%), F4-66 (16.9%) and F1-7 (13.6%). The porB class 3 protein and the fHbp subfamily A (variants 2 and 3) genotypes were found in 93.0 and 71.0% of the isolates, respectively. NHBA was present in all isolates, and while most lacked NadA (94.9%), we detected the hyperinvasive lineages B:P1.19,15:F5-1:ST-639 (cc32); C:P1.22,14-6:F3-9:ST-3780 (cc103) and W:P1.5,2:F1-1:ST-11 (cc11). This is the first report on the genetic diversity and vaccine antigen prevalence among N. meningitidis carriage isolates in the Northeast of Brazil. This study highlights the need for ongoing characterization of meningococcal isolates following the introduction of vaccines and for determining public health intervention strategies.
对从咽部分离出的脑膜炎球菌进行特征分析对于理解脑膜炎球菌携带和疾病的动态变化至关重要。2014年从巴西萨尔瓦多的青少年中收集的脑膜炎球菌分离株，通过多位点序列分型、基因分型或全基因组测序进行特征分析。大多数分离株无法分型（61.0%），其次是B群（11.9%）和Y群（8.5%）。我们鉴定出34种不同的序列类型（STs），其中8种是新的STs，分布在14个克隆复合体（cc）中，cc1136占无法分型分离株的20.3%。porA和fetA基因型包括P1.18,25 - 37（11.9%）、P1.18 - 1,3（10.2%）；F5 - 5（23.7%）、F4 - 66（16.9%）和F1 - 7（13.6%）。分别在93.0%和71.0%的分离株中发现了porB 3类蛋白和fHbp亚家族A（变体2和3）基因型。所有分离株均存在NHBA，虽然大多数缺乏NadA（94.9%），但我们检测到高侵袭性谱系B:P1.19,15:F5 - 1:ST - 639（cc32）；C:P1.22,14 - 6:F3 - 9:ST - 3780（cc103）和W:P1.5,2:F1 - 1:ST - 11（cc11）。这是关于巴西东北部脑膜炎奈瑟菌携带分离株的遗传多样性和疫苗抗原流行情况的首次报告。这项研究强调了在引入疫苗后持续对脑膜炎球菌分离株进行特征分析以及确定公共卫生干预策略的必要性。
