Moreno Jaime, Hidalgo Melissa, Duarte Carolina, Sanabria Olga, Gabastou Jean Marc, Ibarz-Pavon Ana Belén
Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, Colombia.
Pan-American Health Organization, Washington DC, United States of America.
PLoS One. 2015 Aug 31;10(8):e0135497. doi: 10.1371/journal.pone.0135497. eCollection 2015.
Meningococcal carriage studies are important to improve our understanding of the epidemiology of meningococcal disease. The aim of this study was to determine the prevalence of meningococcal carriage and the phenotypic and genotypic characteristics of isolates collected from a sample of students in the city of Bogotá, Colombia.
A total of 1459 oropharyngeal samples were collected from students aged 15-21 years attending secondary schools and universities. Swabs were plated on a Thayer Martin agar and N. meningitidis was identified by standard microbiology methods and PCR.
The overall carriage prevalence was 6.85%. Carriage was associated with cohabitation with smokers, and oral sex practices. Non-groupable and serogroup Y isolates were the most common capsule types found. Isolates presented a high genetic diversity, and circulation of the hypervirulent clonal complexes ST-23, ST-32 and ST-41/44 were detected.
The meningococcal carriage rate was lower than those reported in Europe and Africa, but higher than in other Latin American countries. Our data also revealed antigenic and genetic diversity of the isolates and the circulation of strains belonging to clonal complexes commonly associated with meningococcal disease.
脑膜炎球菌携带情况研究对于增进我们对脑膜炎球菌病流行病学的了解至关重要。本研究的目的是确定哥伦比亚波哥大市学生样本中脑膜炎球菌的携带率以及分离株的表型和基因型特征。
从15至21岁的中学生和大学生中总共采集了1459份口咽样本。将拭子接种在Thayer Martin琼脂上，通过标准微生物学方法和PCR鉴定脑膜炎奈瑟菌。
总体携带率为6.85%。携带情况与与吸烟者同居以及口交行为有关。不可分组和Y血清群分离株是最常见的荚膜类型。分离株呈现出高度的遗传多样性，检测到高毒力克隆复合体ST-23、ST-32和ST-41/44的传播。
脑膜炎球菌携带率低于欧洲和非洲报道的水平，但高于其他拉丁美洲国家。我们的数据还揭示了分离株的抗原和遗传多样性以及属于通常与脑膜炎球菌病相关的克隆复合体的菌株的传播情况。
