Department of Cardiology, Gifu University Graduate School of Medicine.
Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine.
Circ J. 2018 Jan 25;82(2):561-571. doi: 10.1253/circj.CJ-17-0552. Epub 2017 Sep 20.
Multilineage differentiating stress-enduring (Muse) cells are SSEA3and CD105double-positive pluripotent-like stem cells. We aimed to examine the mobilization of Muse cells into peripheral blood after acute myocardial infarction (AMI) and their effects on left ventricular (LV) function and remodeling.Methods and Results:In 79 patients with AMI, 44 patients with coronary artery disease (CAD), and 64 normal subjects (Control), we measured the number of Muse cells in the peripheral blood by fluorescence-activated cell sorting. Muse cells were measured on days 0, 1, 7, 14, and 21 after AMI. Plasma sphingosine-1-phosphate (S1P) levels were measured. Cardiac echocardiography was performed in the acute (within 7 days) and chronic (6 months) phases of AMI. Muse cell number on day 1 was significantly higher in the AMI (276±137 cells/100 μL) than in the CAD (167±89 cells/100 μL) and Control (164±125 cells/100 μL) groups. Muse cell number peaked on day 1, and had gradually decreased on day 21. Muse cell number positively correlated with plasma S1P levels. Patients with a higher increase in the number of Muse cells in the peripheral blood but not those with a lower increase in number of Muse cells in the acute phase showed improved LV function and remodeling in the chronic phase.
Endogenous Muse cells were mobilized into the peripheral blood after AMI. The number of Muse cells could be a predictor of prognosis in patients with AMI.
多谱系分化应激耐受（Muse）细胞是 SSEA3 和 CD105 双阳性的多能样干细胞。我们旨在研究急性心肌梗死（AMI）后 Muse 细胞向外周血的动员及其对左心室（LV）功能和重构的影响。
在 79 例 AMI 患者、44 例冠心病（CAD）患者和 64 例正常对照者中，我们通过荧光激活细胞分选检测外周血中 Muse 细胞的数量。在 AMI 后第 0、1、7、14 和 21 天测量 Muse 细胞。测量血浆鞘氨醇-1-磷酸（S1P）水平。在 AMI 的急性期（7 天内）和慢性期（6 个月）进行心脏超声心动图检查。AMI 患者第 1 天 Muse 细胞数（276±137 个/100μL）明显高于 CAD 患者（167±89 个/100μL）和正常对照组（164±125 个/100μL）。Muse 细胞数于第 1 天达到峰值，随后逐渐减少至第 21 天。Muse 细胞数与血浆 S1P 水平呈正相关。外周血 Muse 细胞数量增加较高的患者，而非急性期 Muse 细胞数量增加较低的患者，在慢性期 LV 功能和重构得到改善。
AMI 后内源性 Muse 细胞动员至外周血。Muse 细胞数量可能是 AMI 患者预后的预测指标。
