School of Environmental Science and Engineering, Shanghai Jiao Tong University, Minhang District, Shanghai, 200240, China.
East China Sea Fisheries Research Institute, Yangpu District, Shanghai, 200082, China.
Sci Rep. 2017 Sep 20;7(1):12031. doi: 10.1038/s41598-017-12339-x.
Extensive polybrominated diphenyl ethers (PBDEs) use has resulted in its increasingly widespread presence in the environment. PBDEs release from existing products can still persist and accumulate in the environment as well as in human and wildlife magnifying through the food web. Due to its ultra-trace amount in the environment, a novel ultrasensitive nano-rt-iPCR assay has been developed to determine polybrominated diphenyl ethers in natural samples. Numerous amino-DNA and polyclonal antibody (anti-PBDE) were immobilized onto the single-walled carbon nanotubes (SWCNTs) to form antibody-SWCNTs-DNA signal amplifier used in the proposed immunoassay system. Compared with rt-iPCR, this nano-rt-iPCR assay had a higher ratio of signal DNA, which meant higher signal measured and lower detection limit. This proposed nano-rt-iPCR assay was used to determine PBDEs in water samples ranging from 0.5 pg/L to 0.5ng/L; giving the LOD 1 pg/L. To the best of our knowledge, this nano-rt-iPCR is the most sensitive method for PBDEs detection. Because of that, this method needs no pre-concentration or extractions, using sample sizes as low as 10 µL. In general, this nano-rt-iPCR method will be a useful and potential way for batch detection of ultra-trace PBDEs in the aquatic environment.
广泛使用多溴二苯醚（PBDEs）导致其在环境中越来越广泛存在。现有产品释放的 PBDEs 仍然可以在环境中以及人类和野生动物中持续存在和积累，并通过食物链放大。由于其在环境中的痕量含量，开发了一种新颖的超灵敏纳米实时聚合酶链反应（rt-iPCR）测定法，用于测定天然样品中的多溴二苯醚。许多氨基-DNA 和多克隆抗体（抗-PBDE）被固定在单壁碳纳米管（SWCNTs）上，形成用于拟议免疫测定系统的抗体-SWCNTs-DNA 信号放大器。与 rt-iPCR 相比，这种纳米 rt-iPCR 测定法具有更高的信号 DNA 比例，这意味着更高的信号测量值和更低的检测限。该纳米 rt-iPCR 测定法用于测定 0.5pg/L 至 0.5ng/L 范围内的水样中的 PBDEs；LOD 为 1pg/L。据我们所知，这是检测 PBDEs 的最灵敏方法。正因为如此，该方法无需预浓缩或提取，只需使用低至 10μL 的样品量。总的来说，这种纳米 rt-iPCR 方法将是批量检测水环境中超痕量 PBDEs 的一种有用且有潜力的方法。
