Moein Soheila, Qujeq Durdi, Vaghari Tabari Mostafa, Kashifard Mehrdad, Hajian-Tilaki Karimollah
Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Department of Biochemistry, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Caspian J Intern Med. 2017 Summer;8(3):178-182. doi: 10.22088/cjim.8.3.178.
Inflammatory bowel disease (IBD) involves chronic inflammation of the digestive tract. In the past decades, fecal calprotectin has been proposed as a useful biomarker for the differential diagnosis between IBD patients and healthy controls. We designed this study to evaluate the diagnostic ability of fecal calprotectin (FC) and conventional inflammatory markers in IBD patients.
Thirty patients who underwent colonoscopy were cases and thirty as part of a medical check-up were the controls. These 2 evaluated with regard to and . Severity of the disease was evaluated based on disease endoscopic index. FC, Cross reactive protein (CRP) and Erythrocyte sedimentation rate (ESR) were measured using ELISA, colorimetric and Westergren methods, respectively. The obtained data were analyzed by independent test, correlation test and receiver operating characteristic (ROC) curve analysis. A p<0.05 was considered statistically significant.
Fecal calprotectin level in the case group increased compared to control group (p<0.05). Moreover FC has stronger correlation with disease endoscopic activity than conventional inflammatory markers (r=0.847 versus r= -0.44 for CRP and r=0.054 for ESR in Crohn's disease and r=0.798 versus r=0.463 for CRP and r=0.467 for ESR in ulcerative colitis). Receiver operating characteristic (ROC) curve analysis showed FC has larger area under the curve (AUC) than conventional inflammatory markers (1 versus 0.849 for CRP and 0.846 for ESR).
Discriminating IBD patients from healthy controls was better for FC than conventional inflammatory markers. Additionally, the results produced by FC correlate with the severity of IBD.
炎症性肠病（IBD）涉及消化道的慢性炎症。在过去几十年中，粪便钙卫蛋白已被提议作为IBD患者与健康对照之间鉴别诊断的有用生物标志物。我们设计了这项研究来评估粪便钙卫蛋白（FC）和传统炎症标志物在IBD患者中的诊断能力。
30例行结肠镜检查的患者为病例组，30名作为体检一部分的患者为对照组。这两组在……方面进行评估。根据疾病内镜指数评估疾病严重程度。分别使用酶联免疫吸附测定法（ELISA）、比色法和魏氏法测量FC、C反应蛋白（CRP）和红细胞沉降率（ESR）。通过独立检验、相关性检验和受试者工作特征（ROC）曲线分析对获得的数据进行分析。p<0.05被认为具有统计学意义。
病例组的粪便钙卫蛋白水平高于对照组（p<0.05）。此外，与传统炎症标志物相比，FC与疾病内镜活动的相关性更强（在克罗恩病中，FC的r = 0.847，而CRP的r = -0.44，ESR的r = 0.054；在溃疡性结肠炎中，FC的r = 0.798，而CRP的r = 0.463，ESR的r = 0.467）。受试者工作特征（ROC）曲线分析显示，FC的曲线下面积（AUC）大于传统炎症标志物（CRP的AUC为0.849，ESR的AUC为0.846，FC的AUC为1）。
与传统炎症标志物相比，FC在区分IBD患者与健康对照方面表现更佳。此外，FC产生的结果与IBD的严重程度相关。
