Shah Anant, Bush Nigel, Box Gary, Eccles Suzanne, Bamber Jeffrey
The Institute of Cancer Research and Royal Marsden NHS Foundation Trust, Joint Department of Physics and CRUK Cancer Imaging Centre in the Division of Radiotherapy and Imaging - Sutton, United Kingdom.
The Institute of Cancer Research, Division of Cancer Therapeutics - Sutton, United Kingdom.
Photoacoustics. 2017 Sep 7;8:15-27. doi: 10.1016/j.pacs.2017.08.001. eCollection 2017 Dec.
Optoacoustic imaging (OAI) can detect haemoglobin and assess its oxygenation. However, the lack of a haemoglobin signal need not indicate a lack of perfusion. This study uses a novel method to assist the co-registration of optoacoustic images with dynamic contrast enhanced ultrasound (DCE-US) images to demonstrate, in preclinical tumour models, the value of combining haemoglobin imaging with a perfusion imaging method, showing that a lack of a haemoglobin signal does not necessarily indicate an absence of perfusion. DCE-US was chosen for this particular experiment because US is extremely sensitive to microbubble contrast agents and because microbubbles, like red blood cells but unlike currently available optical contrast agents, do not extravasate. Significant spatial correlations were revealed between the DCE-US properties and tumour blood-oxygen saturation and haemoglobin, as estimated using OAI. It is speculated that DCE-US properties could be applied as surrogate biomarkers for hypoxia when planning clinical radiotherapy or chemotherapy.
光声成像（OAI）能够检测血红蛋白并评估其氧合状态。然而，缺乏血红蛋白信号并不一定意味着缺乏灌注。本研究采用一种新方法，辅助光声图像与动态对比增强超声（DCE-US）图像的配准，以在临床前肿瘤模型中证明，将血红蛋白成像与一种灌注成像方法相结合的价值，表明缺乏血红蛋白信号不一定表明没有灌注。选择DCE-US进行这个特定实验，是因为超声对微泡造影剂极其敏感，而且微泡与红细胞一样，但与目前可用的光学造影剂不同，不会渗出。如通过OAI估计的那样，DCE-US特性与肿瘤血氧饱和度及血红蛋白之间显示出显著的空间相关性。据推测，在规划临床放疗或化疗时，DCE-US特性可用作缺氧的替代生物标志物。
